Simian Hemorrhagic Fever Virus Cell Entry Is Dependent on CD163 and Uses a Clathrin-Mediated Endocytosis-Like Pathway

  1. Caì, Yíngyún
  2. Postnikova, Elena N.
  3. Bernbaum, John G.
  4. Yú, Shuǐqìng
  5. Mazur, Steven
  6. Deiuliis, Nicole M.
  7. Radoshitzky, Sheli R.
  8. Lackemeyer, Matthew G.
  9. McCluskey, Adam
  10. Robinson, Phillip J.
  11. Wahl-Jensen, Victoria
  12. Bailey, Adam L.
  13. Lauck, Michael
  14. Friedrich, Thomas C.
  15. O'Connor, David H.
  16. Goldberg, Tony L.
  17. Jahrling, Peter B.
  18. Kuhn, Jens H.
  19. Haucke, Volker ORCID logo

WeightNameValue
1000 Titel
  • Simian Hemorrhagic Fever Virus Cell Entry Is Dependent on CD163 and Uses a Clathrin-Mediated Endocytosis-Like Pathway
1000 Autor/in
  1. Caì, Yíngyún |
  2. Postnikova, Elena N. |
  3. Bernbaum, John G. |
  4. Yú, Shuǐqìng |
  5. Mazur, Steven |
  6. Deiuliis, Nicole M. |
  7. Radoshitzky, Sheli R. |
  8. Lackemeyer, Matthew G. |
  9. McCluskey, Adam |
  10. Robinson, Phillip J. |
  11. Wahl-Jensen, Victoria |
  12. Bailey, Adam L. |
  13. Lauck, Michael |
  14. Friedrich, Thomas C. |
  15. O'Connor, David H. |
  16. Goldberg, Tony L. |
  17. Jahrling, Peter B. |
  18. Kuhn, Jens H. |
  19. Haucke, Volker |
1000 Erscheinungsjahr 2014
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2014-10-29
1000 Erschienen in
1000 Quellenangabe
  • 89(1): 844-856
1000 FRL-Sammlung
1000 Verlagsversion
  • http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4301170/ |
  • http://dx.doi.org/10.1128/JVI.02697-14 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Simian hemorrhagic fever virus (SHFV) causes a severe and almost uniformly fatal viral hemorrhagic fever in Asian macaques but is thought to be nonpathogenic for humans. To date, the SHFV life cycle is almost completely uncharacterized on the molecular level. Here, we describe the first steps of the SHFV life cycle. Our experiments indicate that SHFV enters target cells by low-pH-dependent endocytosis. Dynamin inhibitors, chlorpromazine, methyl-β-cyclodextrin, chloroquine, and concanamycin A dramatically reduced SHFV entry efficiency, whereas the macropinocytosis inhibitors EIPA, blebbistatin, and wortmannin and the caveolin-mediated endocytosis inhibitors nystatin and filipin III had no effect. Furthermore, overexpression and knockout study and electron microscopy results indicate that SHFV entry occurs by a dynamin-dependent clathrin-mediated endocytosis-like pathway. Experiments utilizing latrunculin B, cytochalasin B, and cytochalasin D indicate that SHFV does not hijack the actin polymerization pathway. Treatment of target cells with proteases (proteinase K, papain, α-chymotrypsin, and trypsin) abrogated entry, indicating that the SHFV cell surface receptor is a protein. Phospholipases A2 and D had no effect on SHFV entry. Finally, treatment of cells with antibodies targeting CD163, a cell surface molecule identified as an entry factor for the SHFV-related porcine reproductive and respiratory syndrome virus, diminished SHFV replication, identifying CD163 as an important SHFV entry component.
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  1. https://frl.publisso.de/adhoc/creator/Q2HDrCwgWcOtbmd5w7pu|https://frl.publisso.de/adhoc/creator/UG9zdG5pa292YSwgRWxlbmEgTi4=|https://frl.publisso.de/adhoc/creator/QmVybmJhdW0sIEpvaG4gRy4=|https://frl.publisso.de/adhoc/creator/WcO6LCBTaHXHkHHDrG5n|https://frl.publisso.de/adhoc/creator/TWF6dXIsIFN0ZXZlbg==|https://frl.publisso.de/adhoc/creator/RGVpdWxpaXMsIE5pY29sZSBNLg==|https://frl.publisso.de/adhoc/creator/UmFkb3NoaXR6a3ksIFNoZWxpIFIu|https://frl.publisso.de/adhoc/creator/TGFja2VtZXllciwgTWF0dGhldyBHLg==|https://frl.publisso.de/adhoc/creator/TWNDbHVza2V5LCBBZGFt|https://frl.publisso.de/adhoc/creator/Um9iaW5zb24sIFBoaWxsaXAgSi4=|https://frl.publisso.de/adhoc/creator/V2FobC1KZW5zZW4sIFZpY3Rvcmlh|https://frl.publisso.de/adhoc/creator/QmFpbGV5LCBBZGFtIEwu|https://frl.publisso.de/adhoc/creator/TGF1Y2ssIE1pY2hhZWw=|https://frl.publisso.de/adhoc/creator/RnJpZWRyaWNoLCBUaG9tYXMgQy4=|https://frl.publisso.de/adhoc/creator/TydDb25ub3IsIERhdmlkIEgu|https://frl.publisso.de/adhoc/creator/R29sZGJlcmcsIFRvbnkgTC4=|https://frl.publisso.de/adhoc/creator/SmFocmxpbmcsIFBldGVyIEIu|https://frl.publisso.de/adhoc/creator/S3VobiwgSmVucyBILg==|http://orcid.org/0000-0003-3119-6993
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