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WeightNameValue
1000 Titel
  • Cultured bovine granulosa cells rapidly lose important features of their identity and functionality but partially recover under long-term culture conditions
1000 Autor/in
  1. Yenuganti, Vengala Rao |
  2. Vanselow, Jens |
1000 Erscheinungsjahr 2017
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-02-02
1000 Erschienen in
1000 Quellenangabe
  • 368(2): 397–403
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2017
1000 Lizenz
1000 Verlagsversion
  • http://dx.doi.org/10.1007/s00441-017-2571-6 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5397658/ |
1000 Ergänzendes Material
  • https://link.springer.com/article/10.1007%2Fs00441-017-2571-6#SupplementaryMaterial |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Cell culture models are essential for the detailed study of molecular processes. We analyze the dynamics of changes in a culture model of bovine granulosa cells. The cells were cultured for up to 8 days and analyzed for steroid production and gene expression. According to the expression of the marker genes CDH1, CDH2 and VIM, the cells maintained their mesenchymal character throughout the time of culture. In contrast, the levels of functionally important transcripts and of estradiol and progesterone production were rapidly down-regulated but showed a substantial up-regulation from day 4. FOXL2, a marker for granulosa cell identity, was also rapidly down-regulated after plating but completely recovered towards the end of culture. In contrast, expression of the Sertoli cell marker SOX9 and the lesion/inflammation marker PTGS2 increased during the first 2 days after plating but gradually decreased later on. We conclude that only long-term culture conditions (>4 days) allow the cells to recover from plating stress and to re-acquire characteristic granulosa cell features.
1000 Sacherschließung
lokal Cell identity
lokal FOXL2
lokal PTGS2
lokal SOX9
lokal Cell plating
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/WWVudWdhbnRpLCBWZW5nYWxhIFJhbw==|http://orcid.org/0000-0001-7374-4615
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1000 @id frl:6402375.rdf
1000 Erstellt am 2017-05-10T14:09:46.376+0200
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1000 Vgl. frl:6402375
1000 Oai Id
  1. oai:frl.publisso.de:frl:6402375 |
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