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1000
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Abstract/Summary
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Supplementation of micronutrients involved in DNA methylation, particularly during
pregnancy, is recommended because of its impacts on human health, but further evidence is needed
regarding the effects of over-supplementation and differences between sexes. Here, a porcine model
was used to assess effects of maternal supplementation with one-carbon-cycle compounds during
prenatal and postnatal stages on offspring muscle development. Sows received either a standard diet
(CON) or a standard diet supplemented with folate, B6, B12, methionine, choline, and zinc (MET)
throughout gestation. Myogenesis-, growth-, and nutrient utilization-related transcript expression
was assessed using quantitative PCR. Organismal phenotype and gene expression effects differed
significantly between males and females. Male MET-offspring showed increased fetal weight during
late pregnancy but decreased live weight postnatally, with compensatory transcriptional responses
comprising myogenic key drivers (Pax7, MyoD1, myogenin). In contrast, female weights were
unaffected by diet, and mRNA abundances corresponded to a phenotype of cellular reorganization
via FABP3, FABP4, SPP1 and Insulin-like Growth Factor-signaling. These findings in an animal
model suggest that supplementation during pregnancy with methylation-related micronutrients
can promote sex-specific myogenic maturation processes related to organismal growth and muscle
metabolism. The usage of maternal dietary supplements should be more carefully considered
regarding its ability to promote fetal and postnatal health.
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