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1000 Titel
  • Additive effects on the energy barrier for synaptic vesicle fusion cause supralinear effects on the vesicle fusion rate
1000 Autor/in
  1. Schotten, Sebastiaan |
  2. Meijer, Marieke |
  3. Huson, Vincent |
  4. Mamer, Lauren |
  5. Kalogreades, Lawrence |
  6. ter Veer, Mirelle |
  7. Ruiter, Marvin |
  8. Brose, Nils |
  9. Rosenmund, Christian |
  10. Sørensen, Jakob Balslev |
  11. Verhage, Matthijs |
  12. Cornelisse, Lennart Niels |
  13. Walter, Alexander |
1000 Erscheinungsjahr 2015
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2015-04-14
1000 Erschienen in
1000 Quellenangabe
  • 4: e05531
1000 FRL-Sammlung
1000 Lizenz
1000 Verlagsversion
  • http://dx.doi.org/10.7554/eLife.05531 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4426983/ |
1000 Ergänzendes Material
  • https://elifesciences.org/content/4/e05531/article-data |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The energy required to fuse synaptic vesicles with the plasma membrane (‘activation energy’) is considered a major determinant in synaptic efficacy. From reaction rate theory, we predict that a class of modulations exists, which utilize linear modulation of the energy barrier for fusion to achieve supralinear effects on the fusion rate. To test this prediction experimentally, we developed a method to assess the number of releasable vesicles, rate constants for vesicle priming, unpriming, and fusion, and the activation energy for fusion by fitting a vesicle state model to synaptic responses induced by hypertonic solutions. We show that complexinI/II deficiency or phorbol ester stimulation indeed affects responses to hypertonic solution in a supralinear manner. An additive vs multiplicative relationship between activation energy and fusion rate provides a novel explanation for previously observed non-linear effects of genetic/pharmacological perturbations on synaptic transmission and a novel interpretation of the cooperative nature of Ca2+-dependent release.
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/U2Nob3R0ZW4sIFNlYmFzdGlhYW4=|https://frl.publisso.de/adhoc/creator/TWVpamVyLCBNYXJpZWtl|https://frl.publisso.de/adhoc/creator/SHVzb24sIFZpbmNlbnQ=|https://frl.publisso.de/adhoc/creator/TWFtZXIsIExhdXJlbg==|https://frl.publisso.de/adhoc/creator/S2Fsb2dyZWFkZXMsIExhd3JlbmNl|https://frl.publisso.de/adhoc/creator/dGVyIFZlZXIsIE1pcmVsbGU=|https://frl.publisso.de/adhoc/creator/UnVpdGVyLCBNYXJ2aW4=|https://frl.publisso.de/adhoc/creator/QnJvc2UsIE5pbHM=|https://frl.publisso.de/adhoc/creator/Um9zZW5tdW5kLCBDaHJpc3RpYW4=|https://frl.publisso.de/adhoc/creator/U8O4cmVuc2VuLCBKYWtvYiBCYWxzbGV2|https://frl.publisso.de/adhoc/creator/VmVyaGFnZSwgTWF0dGhpanM=|https://frl.publisso.de/adhoc/creator/Q29ybmVsaXNzZSwgTGVubmFydCBOaWVscw==|http://orcid.org/0000-0001-5646-4750
1000 Förderer
  1. Nederlandse Organisatie voor Wetenschappelijk Onderzoek |
  2. Fourth Framework Programme (European Union Fourth Framework Programme) |
  3. European Commission |
1000 Fördernummer
  1. CLS2007 project 635100020; Complexity project 645.000.003; TOP project 91207032
  2. Synsys Health-F4-2010- 242167; Eurospin Health- F2-2009-241498
  3. Eurospin Health-F2-2009-241498
1000 Förderprogramm
  1. -
  2. -
  3. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Nederlandse Organisatie voor Wetenschappelijk Onderzoek |
    1000 Förderprogramm -
    1000 Fördernummer CLS2007 project 635100020; Complexity project 645.000.003; TOP project 91207032
  2. 1000 joinedFunding-child
    1000 Förderer Fourth Framework Programme (European Union Fourth Framework Programme) |
    1000 Förderprogramm -
    1000 Fördernummer Synsys Health-F4-2010- 242167; Eurospin Health- F2-2009-241498
  3. 1000 joinedFunding-child
    1000 Förderer European Commission |
    1000 Förderprogramm -
    1000 Fördernummer Eurospin Health-F2-2009-241498
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6402671.rdf
1000 Erstellt am 2017-05-31T12:25:42.830+0200
1000 Erstellt von 24
1000 beschreibt frl:6402671
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Tue Jul 31 12:04:46 CEST 2018
1000 Objekt bearb. Tue Jul 31 12:04:46 CEST 2018
1000 Vgl. frl:6402671
1000 Oai Id
  1. oai:frl.publisso.de:frl:6402671 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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