WeightNameValue
1000 Titel
  • Inositol polyphosphates intersect with signaling and metabolic networks via two distinct mechanisms
1000 Autor/in
  1. Wu, Mingxuan |
  2. Chong, Lucy S. |
  3. Perlman, David H. |
  4. Resnick, Adam C. |
  5. Fiedler, Dorothea |
1000 Erscheinungsjahr 2016
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-11-01
1000 Erschienen in
1000 Quellenangabe
  • 113(44): E6757–E6765
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5098652/ |
  • http://doi.org/10.1073/pnas.1606853113 |
1000 Ergänzendes Material
  • http://www.pnas.org/content/suppl/2016/10/18/1606853113.DCSupplemental |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Inositol-based signaling molecules are central eukaryotic messengers and include the highly phosphorylated, diffusible inositol polyphosphates (InsPs) and inositol pyrophosphates (PP-InsPs). Despite the essential cellular regulatory functions of InsPs and PP-InsPs (including telomere maintenance, phosphate sensing, cell migration, and insulin secretion), the majority of their protein targets remain unknown. Here, the development of InsP and PP-InsP affinity reagents is described to comprehensively annotate the interactome of these messenger molecules. By using the reagents as bait, >150 putative protein targets were discovered from a eukaryotic cell lysate (Saccharomyces cerevisiae). Gene Ontology analysis of the binding partners revealed a significant overrepresentation of proteins involved in nucleotide metabolism, glucose metabolism, ribosome biogenesis, and phosphorylation-based signal transduction pathways. Notably, we isolated and characterized additional substrates of protein pyrophosphorylation, a unique posttranslational modification mediated by the PP-InsPs. Our findings not only demonstrate that the PP-InsPs provide a central line of communication between signaling and metabolic networks, but also highlight the unusual ability of these molecules to access two distinct modes of action.
1000 Sacherschließung
lokal affinity reagents
lokal protein pyrophosphorylation
lokal signal transduction
lokal inositol pyrophosphates
lokal metabolism
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/V3UsIE1pbmd4dWFu|https://frl.publisso.de/adhoc/creator/Q2hvbmcsIEx1Y3kgUy4=|https://frl.publisso.de/adhoc/creator/UGVybG1hbiwgRGF2aWQgSC4=|https://frl.publisso.de/adhoc/creator/UmVzbmljaywgQWRhbSBDLg==|https://frl.publisso.de/adhoc/creator/RmllZGxlciwgRG9yb3RoZWE=
1000 Förderer
  1. NIH Grant |
  2. Princeton University |
1000 Fördernummer
  1. R00 GM087306
  2. -
1000 Förderprogramm
  1. -
  2. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer NIH Grant |
    1000 Förderprogramm -
    1000 Fördernummer R00 GM087306
  2. 1000 joinedFunding-child
    1000 Förderer Princeton University |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6402697.rdf
1000 Erstellt am 2017-05-31T15:35:44.890+0200
1000 Erstellt von 25
1000 beschreibt frl:6402697
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Mon May 28 10:08:04 CEST 2018
1000 Objekt bearb. Mon May 28 10:08:03 CEST 2018
1000 Vgl. frl:6402697
1000 Oai Id
  1. oai:frl.publisso.de:frl:6402697 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

View source