RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses

  1. Grauel, M. Katharina
  2. Maglione, Marta
  3. Reddy-Alla, Suneel
  4. Willmes, Claudia G.
  5. Brockmann, Marisa M.
  6. Trimbuch, Thorsten
  7. Rosenmund, Tanja
  8. Pangalos, Maria
  9. Vardar, Gülçin
  10. Stumpf, Alexander
  11. Rost, Benjamin R.
  12. Eickholt, Britta J.
  13. Schmitz, Dietmar
  14. Sigrist, Stephan J.
  15. Rosenmund, Christian
  16. Haucke, Volker ORCID logo
  17. Walter, Alexander ORCID logo

WeightNameValue
1000 Titel
  • RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses
1000 Autor/in
  1. Grauel, M. Katharina |
  2. Maglione, Marta |
  3. Reddy-Alla, Suneel |
  4. Willmes, Claudia G. |
  5. Brockmann, Marisa M. |
  6. Trimbuch, Thorsten |
  7. Rosenmund, Tanja |
  8. Pangalos, Maria |
  9. Vardar, Gülçin |
  10. Stumpf, Alexander |
  11. Rost, Benjamin R. |
  12. Eickholt, Britta J. |
  13. Schmitz, Dietmar |
  14. Sigrist, Stephan J. |
  15. Rosenmund, Christian |
  16. Haucke, Volker |
  17. Walter, Alexander |
1000 Erscheinungsjahr 2016
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-10-11
1000 Erschienen in
1000 Quellenangabe
  • 113(41): 11615–11620
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5068320/ |
  • http://dx.doi.org/10.1073/pnas.1605256113 |
1000 Ergänzendes Material
  • http://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1605256113/-/DCSupplemental |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The tight spatial coupling of synaptic vesicles and voltage-gated Ca2+ channels (CaVs) ensures efficient action potential-triggered neurotransmitter release from presynaptic active zones (AZs). Rab-interacting molecule-binding proteins (RIM-BPs) interact with Ca2+ channels and via RIM with other components of the release machinery. Although human RIM-BPs have been implicated in autism spectrum disorders, little is known about the role of mammalian RIM-BPs in synaptic transmission. We investigated RIM-BP2-deficient murine hippocampal neurons in cultures and slices. Short-term facilitation is significantly enhanced in both model systems. Detailed analysis in culture revealed a reduction in initial release probability, which presumably underlies the increased short-term facilitation. Superresolution microscopy revealed an impairment in CaV2.1 clustering at AZs, which likely alters Ca2+ nanodomains at release sites and thereby affects release probability. Additional deletion of RIM-BP1 does not exacerbate the phenotype, indicating that RIM-BP2 is the dominating RIM-BP isoform at these synapses.
1000 Sacherschließung
lokal Neuroscience
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/R3JhdWVsLCBNLiBLYXRoYXJpbmE=|https://frl.publisso.de/adhoc/creator/TWFnbGlvbmUsIE1hcnRh|https://frl.publisso.de/adhoc/creator/UmVkZHktQWxsYSwgU3VuZWVs|https://frl.publisso.de/adhoc/creator/V2lsbG1lcywgQ2xhdWRpYSBHLg==|https://frl.publisso.de/adhoc/creator/QnJvY2ttYW5uLCBNYXJpc2EgTS4=|https://frl.publisso.de/adhoc/creator/VHJpbWJ1Y2gsIFRob3JzdGVu|https://frl.publisso.de/adhoc/creator/Um9zZW5tdW5kLCBUYW5qYQ==|https://frl.publisso.de/adhoc/creator/UGFuZ2Fsb3MsIE1hcmlh|https://frl.publisso.de/adhoc/creator/VmFyZGFyLCAgR8O8bMOnaW4=|https://frl.publisso.de/adhoc/creator/U3R1bXBmLCBBbGV4YW5kZXI=|https://frl.publisso.de/adhoc/creator/Um9zdCwgQmVuamFtaW4gUi4=|https://frl.publisso.de/adhoc/creator/RWlja2hvbHQsIEJyaXR0YSBKLg==|https://frl.publisso.de/adhoc/creator/U2NobWl0eiwgRGlldG1hcg==|https://frl.publisso.de/adhoc/creator/U2lncmlzdCwgU3RlcGhhbiBKLg==|https://frl.publisso.de/adhoc/creator/Um9zZW5tdW5kLCBDaHJpc3RpYW4=|http://orcid.org/0000-0003-3119-6993|http://orcid.org/0000-0001-5646-4750
1000 (Academic) Editor
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
  2. Emmy-Noether Program |
  3. Studienstiftung des deutschen Volkes PhD Fellowship |
  4. Excellence Cluster Neurocure |
1000 Fördernummer
  1. SFB 958
  2. -
  3. -
  4. Exc257
1000 Förderprogramm
  1. -
  2. -
  3. -
  4. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer SFB 958
  2. 1000 joinedFunding-child
    1000 Förderer Emmy-Noether Program |
    1000 Förderprogramm -
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Studienstiftung des deutschen Volkes PhD Fellowship |
    1000 Förderprogramm -
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer Excellence Cluster Neurocure |
    1000 Förderprogramm -
    1000 Fördernummer Exc257
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6402731.rdf
1000 Erstellt am 2017-06-01T14:13:19.761+0200
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1000 Bearbeitet von 218
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1000 Oai Id
  1. oai:frl.publisso.de:frl:6402731 |
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