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WeightNameValue
1000 Titel
  • Integrated Assessment and Prediction of Transcription Factor Binding
1000 Autor/in
  1. Beyer, Andreas |
  2. Workman, Christopher |
  3. Hollunder, Jens |
  4. Radke, Dörte |
  5. Möller, Ulrich |
  6. Wilhelm, Thomas |
  7. Ideker, Trey |
1000 Erscheinungsjahr 2006
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2006-06-16
1000 Erschienen in
1000 Quellenangabe
  • 2(6): e70
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2006
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1371/journal.pcbi.0020070 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1479087/ |
1000 Ergänzendes Material
  • http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.0020070#s4 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Systematic chromatin immunoprecipitation (chIP-chip) experiments have become a central technique for mapping transcriptional interactions in model organisms and humans. However, measurement of chromatin binding does not necessarily imply regulation, and binding may be difficult to detect if it is condition or cofactor dependent. To address these challenges, we present an approach for reliably assigning transcription factors (TFs) to target genes that integrates many lines of direct and indirect evidence into a single probabilistic model. Using this approach, we analyze publicly available chIP-chip binding profiles measured for yeast TFs in standard conditions, showing that our model interprets these data with significantly higher accuracy than previous methods. Pooling the high-confidence interactions reveals a large network containing 363 significant sets of factors (TF modules) that cooperate to regulate common target genes. In addition, the method predicts 980 novel binding interactions with high confidence that are likely to occur in so-far untested conditions. Indeed, using new chIP-chip experiments we show that predicted interactions for the factors Rpn4p and Pdr1p are observed only after treatment of cells with methyl-methanesulfonate, a DNA-damaging agent. We outline the first approach for consistently integrating all available evidences for TF–target interactions and we comprehensively identify the resulting TF module hierarchy. Prioritizing experimental conditions for each factor will be especially important as increasing numbers of chIP-chip assays are performed in complex organisms such as humans, for which “standard conditions” are ill defined.
1000 Sacherschließung
lokal Cell binding
lokal Genetic networks
lokal Transcription factors
lokal Binding analysis
lokal DNA
lokal Gene regulation
lokal Gene expression
lokal Saccharomyces cerevisiae
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. http://orcid.org/0000-0002-3891-2123|https://frl.publisso.de/adhoc/creator/V29ya21hbiwgQ2hyaXN0b3BoZXI=|http://orcid.org/0000-0003-3234-6762|https://frl.publisso.de/adhoc/creator/UmFka2UsIETDtnJ0ZQ==|https://frl.publisso.de/adhoc/creator/TcO2bGxlciwgVWxyaWNo|https://frl.publisso.de/adhoc/creator/V2lsaGVsbSwgVGhvbWFz|https://frl.publisso.de/adhoc/creator/SWRla2VyLCBUcmV5
1000 Förderer
  1. German Academic Exchange Service |
  2. German Federal Ministry for Education and Research (BMBF) |
1000 Fördernummer
  1. -
  2. 0312704E
1000 Förderprogramm
  1. -
  2. -
1000 Dateien
  1. Integrated Assessment and Prediction of Transcription Factor Binding
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Academic Exchange Service |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer German Federal Ministry for Education and Research (BMBF) |
    1000 Förderprogramm -
    1000 Fördernummer 0312704E
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6402930.rdf
1000 Erstellt am 2017-06-08T08:52:54.051+0200
1000 Erstellt von 21
1000 beschreibt frl:6402930
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Mon May 28 10:12:24 CEST 2018
1000 Objekt bearb. Mon May 28 10:12:24 CEST 2018
1000 Vgl. frl:6402930
1000 Oai Id
  1. oai:frl.publisso.de:frl:6402930 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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