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1000 Titel
  • A translation control reporter system (TCRS) for the analysis of translationally controlled processes in the vertebrate cell
1000 Autor/in
  1. Wiesenthal, Volker |
  2. Leutz, Achim |
  3. Calkhoven, Cornelis F. |
1000 Erscheinungsjahr 2006
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2006-01-01
1000 Erschienen in
1000 Quellenangabe
  • 34(3): e23
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2006
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1093/nar/gnj029 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1363784/ |
1000 Ergänzendes Material
  • https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gnj029#33512761 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Regulation of translation is critical for the accurate expression of a broad variety of genes that function in cell cycle progression and cell differentiation, as well as in the adaptation to cellular stress. The aetiologies of a number of human diseases, including cancer, have been linked to mutations in genes that control mRNA translation, or in cis-regulatory mRNA-sequences. Therefore, research on translational control and its therapeutic appliance has become most important. However, to date only a limited number of therapeutic drugs are known to affect translational control. Here we describe a novel, straightforward approach for the detection of cellular translational activity. We developed a Translational Control Reporter System (TCRS), which utilizes the cis-regulatory upstream open reading frame (uORF) from the c/ebpα locus to direct the translation of a dual reporter gene into two unique reporter peptides. The peptides contain a pre-pro-trypsin (PPT) signal for secretion into the medium and distinct immunogenic epitopes for detection and quantification purposes. TCRS-peptide expression levels reflect changes of translation initiation induced by serum growth factors, drugs or translation factor mutants. TCRS can be tailored to various research settings and the system may accomplish a broad application to uncover links between translational control and drugs.
1000 Sacherschließung
lokal Transcriptome Mapping - Monitoring Gene Expression
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/V2llc2VudGhhbCwgVm9sa2Vy|https://frl.publisso.de/adhoc/creator/TGV1dHosIEFjaGlt|https://frl.publisso.de/adhoc/creator/Q2Fsa2hvdmVuLCBDb3JuZWxpcyBGLg==
1000 Förderer
  1. MDC |
  2. DFG |
  3. Leibniz Institute of Age Research - Fritz Lipmann Institute, Jena, Germany |
1000 Fördernummer
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1000 Förderprogramm
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1000 Dateien
1000 Förderung
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    1000 Förderer MDC |
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    1000 Förderer Leibniz Institute of Age Research - Fritz Lipmann Institute, Jena, Germany |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6402936.rdf
1000 Erstellt am 2017-06-08T09:47:27.936+0200
1000 Erstellt von 21
1000 beschreibt frl:6402936
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Mon Apr 30 13:05:27 CEST 2018
1000 Objekt bearb. Mon Apr 30 13:05:27 CEST 2018
1000 Vgl. frl:6402936
1000 Oai Id
  1. oai:frl.publisso.de:frl:6402936 |
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