WeightNameValue
1000 Titel
  • Inhibition of Biosynthesis of Human Endothelin B Receptor by the Cyclodepsipeptide Cotransin
1000 Autor/in
  1. Westendorf, Carolin |
  2. Schmidt, Antje |
  3. Coin, Irene |
  4. Furkert, Jens |
  5. Ridelis, Ingrid |
  6. Zampatis, Dimitris |
  7. Rutz, Claudia |
  8. Wiesner, Burkhard |
  9. Rosenthal, Walter |
  10. Beyermann, Michael |
  11. Schülein, Ralf |
1000 Erscheinungsjahr 2011
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2011-07-30
1000 Erschienen in
1000 Quellenangabe
  • 286: 35588-35600
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3195616/ |
  • http://doi.org/10.1074/jbc.M111.239244 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The specific inhibition of the biosynthesis of target proteins is a relatively novel strategy in pharmacology and is based mainly on antisense approaches (e.g. antisense oligonucleotides or RNA interference). Recently, a novel class of substances was described acting at a later step of protein biosynthesis. The cyclic heptadepsipeptides CAM741 and cotransin were shown to inhibit selectively the biosynthesis of a small subset of secretory proteins by preventing stable insertion of the nascent chains into the Sec61 translocon complex at the endoplasmic reticulum membrane (Besemer, J., Harant, H., Wang, S., Oberhauser, B., Marquardt, K., Foster, C. A., Schreiner, E. P., de Vries, J. E., Dascher-Nadel, C., and Lindley, I. J. (2005) Nature 436, 290–293; Garrison, J. L., Kunkel, E. J., Hegde, R. S., and Taunton, J. (2005) Nature 436, 285–289). These peptides act in a signal sequence-discriminatory manner, which explains their selectivity. Here, we have analyzed the cotransin sensitivity of various G protein-coupled receptors in transfected HEK 293 cells. We show that the biosynthesis of the human endothelin B receptor (ETBR) is highly sensitive to cotransin, in contrast to that of the other G protein-coupled receptors analyzed. Using a novel biosynthesis assay based on fusions with the photoconvertible Kaede protein, we show that the IC50 value of cotransin action on ETBR biosynthesis is 5.4 μM and that ETBR signaling could be completely blocked by treating cells with 30 μM cotransin. Taken together, our data add an integral membrane protein, namely the ETBR, to the small group of cotransin-sensitive proteins.
1000 Sacherschließung
lokal Protein Export
lokal Protein Translocation
lokal Receptor Regulation
lokal G Protein-coupled Receptors (GPCR)
lokal Receptors
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/V2VzdGVuZG9yZiwgQ2Fyb2xpbg==|https://frl.publisso.de/adhoc/creator/U2NobWlkdCwgQW50amU=|https://frl.publisso.de/adhoc/creator/Q29pbiwgSXJlbmU=|https://frl.publisso.de/adhoc/creator/RnVya2VydCwgSmVucw==|https://frl.publisso.de/adhoc/creator/UmlkZWxpcywgSW5ncmlk|https://frl.publisso.de/adhoc/creator/WmFtcGF0aXMsIERpbWl0cmlz|https://frl.publisso.de/adhoc/creator/UnV0eiwgQ2xhdWRpYQ==|https://frl.publisso.de/adhoc/creator/V2llc25lciwgQnVya2hhcmQ=|https://frl.publisso.de/adhoc/creator/Um9zZW50aGFsLCBXYWx0ZXI=|https://frl.publisso.de/adhoc/creator/QmV5ZXJtYW5uLCBNaWNoYWVs|https://frl.publisso.de/adhoc/creator/U2Now7xsZWluLCBSYWxm
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
1000 Fördernummer
  1. SFB 449; SCHU 1116/2-1
1000 Förderprogramm
  1. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer SFB 449; SCHU 1116/2-1
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403003.rdf
1000 Erstellt am 2017-06-13T10:22:53.916+0200
1000 Erstellt von 25
1000 beschreibt frl:6403003
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Mon May 28 10:05:25 CEST 2018
1000 Objekt bearb. Mon May 28 10:05:25 CEST 2018
1000 Vgl. frl:6403003
1000 Oai Id
  1. oai:frl.publisso.de:frl:6403003 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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