Download
journal.pone.0011708.PDF 1,91MB
WeightNameValue
1000 Titel
  • Adhesion and Degranulation Promoting Adapter Protein (ADAP) Is a Central Hub for Phosphotyrosine-Mediated Interactions in T Cells
1000 Autor/in
  1. Sylvester, Marc |
  2. Kliche, Stefanie |
  3. Lange, Sabine |
  4. Geithner, Sabine |
  5. Klemm, Clementine |
  6. Schlosser, Andreas |
  7. Großmann, Arndt |
  8. Stelzl, Ulrich |
  9. Schraven, Burkhart |
  10. Krause, Eberhard |
  11. Freund, Christian |
1000 Erscheinungsjahr 2010
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2010-07-22
1000 Erschienen in
1000 Quellenangabe
  • 5(7): e11708
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2010
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1371/journal.pone.0011708 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2908683/ |
1000 Ergänzendes Material
  • http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0011708#s5 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • TCR stimulation leads to an increase in cellular adhesion among other outcomes. The adhesion and degranulation promoting adapter protein (ADAP) is known to be rapidly phosphorylated after T cell stimulation and relays the TCR signal to adhesion molecules of the integrin family. While three tyrosine phosphorylation sites have been characterized biochemically, the binding capabilities and associated functions of several other potential phosphotyrosine motifs remain unclear. Here, we utilize in vitro phosphorylation and mass spectrometry to map novel phosphotyrosine sites in the C-terminal part of human ADAP (486–783). Individual tyrosines were then mutated to phenylalanine and their relevance for cellular adhesion and migration was tested experimentally. Functionally important tyrosine residues include two sites within the folded hSH3 domains of ADAP and two at the C-terminus. Furthermore, using a peptide pulldown approach in combination with stable isotope labeling in cell culture (SILAC) we identified SLP-76, PLCγ, PIK3R1, Nck, CRK, Gads, and RasGAP as phospho-dependent binding partners of a central YDDV motif of ADAP. The phosphorylation-dependent interaction between ADAP and Nck was confirmed by yeast two-hybrid analysis, immunoprecipitation and binary pulldown experiments, indicating that ADAP directly links integrins to modulators of the cytoskeleton independent of SLP-76.
1000 Sacherschließung
lokal Tyrosine
lokal Phosphorylation
lokal Immunoprecipitation
lokal SH2 domain
lokal T cell receptors
lokal T cells
lokal Protein interactions
lokal Integrins
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/U3lsdmVzdGVyLCBNYXJj|https://frl.publisso.de/adhoc/creator/S2xpY2hlLCBTdGVmYW5pZQ==|https://frl.publisso.de/adhoc/creator/TGFuZ2UsIFNhYmluZQ==|https://frl.publisso.de/adhoc/creator/R2VpdGhuZXIsIFNhYmluZQ==|https://frl.publisso.de/adhoc/creator/S2xlbW0sIENsZW1lbnRpbmU=|https://frl.publisso.de/adhoc/creator/U2NobG9zc2VyLCBBbmRyZWFz|https://frl.publisso.de/adhoc/creator/R3Jvw59tYW5uLCBBcm5kdA==|https://frl.publisso.de/adhoc/creator/U3RlbHpsLCBVbHJpY2g=|https://frl.publisso.de/adhoc/creator/U2NocmF2ZW4sIEJ1cmtoYXJ0|https://frl.publisso.de/adhoc/creator/S3JhdXNlLCBFYmVyaGFyZA==|https://frl.publisso.de/adhoc/creator/RnJldW5kLCBDaHJpc3RpYW4=
1000 Förderer
  1. Max-Planck Society |
  2. Nationales Genom-Forschungs-Netz-plus |
  3. Deutsche Forschungsgemeinschaft |
1000 Fördernummer
  1. -
  2. 01GS08171
  3. SFB740
1000 Förderprogramm
  1. -
  2. NeuroNet TP3
  3. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Max-Planck Society |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Nationales Genom-Forschungs-Netz-plus |
    1000 Förderprogramm NeuroNet TP3
    1000 Fördernummer 01GS08171
  3. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer SFB740
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403165.rdf
1000 Erstellt am 2017-06-19T14:55:48.098+0200
1000 Erstellt von 25
1000 beschreibt frl:6403165
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Wed Aug 01 11:52:04 CEST 2018
1000 Objekt bearb. Wed Aug 01 11:52:03 CEST 2018
1000 Vgl. frl:6403165
1000 Oai Id
  1. oai:frl.publisso.de:frl:6403165 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

View source