WeightNameValue
1000 Titel
  • Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV‑1 Protein Assemblies
1000 Autor/in
  1. Gupta, Rupal |
  2. Lu, Manman |
  3. Hou, Guangjin |
  4. Caporini, Marc A. |
  5. Rosay, Melanie |
  6. Maas, Werner |
  7. Struppe, Jochem |
  8. Suiter, Christopher |
  9. Ahn, Jinwoo |
  10. Byeon, In-Ja L. |
  11. Franks, W. Trent |
  12. Orwick-Rydmark, Marcella |
  13. Bertarello, Andrea |
  14. Oschkinat, Hartmut |
  15. Lesage, Anne |
  16. Pintacuda, Guido |
  17. Gronenborn, Angela M. |
  18. Polenova, Tatyana |
1000 Erscheinungsjahr 2017
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-01-21
1000 Erschienen in
1000 Quellenangabe
  • 120(2): 329-339
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890707/ |
  • https://dx.doi.org/10.1021/acs.jpcb.5b12134 |
1000 Ergänzendes Material
  • http://pubs.acs.org/doi/suppl/10.1021/acs.jpcb.5b12134/suppl_file/jp5b12134_si_001.pdf |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Mature infectious HIV-1 virions contain conical capsids comprised of CA protein, generated by the proteolytic cleavage cascade of the Gag polyprotein, termed maturation. The mechanism of capsid core formation through the maturation process remains poorly understood. We present DNP-enhanced MAS NMR studies of tubular assemblies of CA and Gag CA-SP1 maturation intermediate and report 20 – 64 fold sensitivity enhancements due to DNP at 14.1 T. These sensitivity enhancements enabled direct observation of spacer peptide 1 (SP1) resonances in CA-SP1 by dipolar based correlation experiments, unequivocally indicating that the SP1 peptide is unstructured in assembled CA-SP1 at cryogenic temperatures, corroborating our earlier results. Furthermore, the dependence of DNP enhancements and spectral resolution on magnetic field strength (9.4 – 18.8 T) and temperature (109 – 180 K) was investigated. Our results suggest that DNP-based measurements could potentially provide residue-specific dynamics information by allowing for the extraction of temperature dependence of the anisotropic tensorial or relaxation parameters. With DNP, we were able to detect multiple well-resolved isoleucine sidechain conformers, unique intermolecular correlations across two CA molecules, and functionally relevant conformationally disordered states such as the 14-residue SP1 peptide, none of which are visible at ambient temperatures. The detection of isolated conformers and intermolecular correlations can provide crucial constraints for structure determination of these assemblies. Overall, our results establish DNP-based MAS NMR as an excellent tool for characterization of HIV-1 assemblies.
1000 Fachgruppe
  1. Medizin |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/R3VwdGEsIFJ1cGFs|https://frl.publisso.de/adhoc/creator/THUsIE1hbm1hbg==|https://frl.publisso.de/adhoc/creator/SG91LCBHdWFuZ2ppbg==|https://frl.publisso.de/adhoc/creator/Q2Fwb3JpbmksIE1hcmMgQS4=|https://frl.publisso.de/adhoc/creator/Um9zYXksIE1lbGFuaWU=|https://frl.publisso.de/adhoc/creator/TWFhcywgV2VybmVy|https://frl.publisso.de/adhoc/creator/U3RydXBwZSwgSm9jaGVt|https://frl.publisso.de/adhoc/creator/U3VpdGVyLCBDaHJpc3RvcGhlcg==|https://frl.publisso.de/adhoc/creator/QWhuLCBKaW53b28=|https://frl.publisso.de/adhoc/creator/Qnllb24sIEluLUphIEwu|https://frl.publisso.de/adhoc/creator/RnJhbmtzLCBXLiBUcmVudA==|https://frl.publisso.de/adhoc/creator/T3J3aWNrLVJ5ZG1hcmssIE1hcmNlbGxh|https://frl.publisso.de/adhoc/creator/QmVydGFyZWxsbywgQW5kcmVh|https://frl.publisso.de/adhoc/creator/T3NjaGtpbmF0LCBIYXJ0bXV0|https://frl.publisso.de/adhoc/creator/TGVzYWdlLCBBbm5l|https://frl.publisso.de/adhoc/creator/UGludGFjdWRhLCBHdWlkbw==|https://frl.publisso.de/adhoc/creator/R3JvbmVuYm9ybiwgQW5nZWxhIE0u|https://frl.publisso.de/adhoc/creator/UG9sZW5vdmEsIFRhdHlhbmE=
1000 Förderer
  1. National Institute of Health |
  2. NSF |
  3. NIGMS |
  4. EQUIPEX |
  5. IR-RMN-THC FRE |
  6. European Union |
1000 Fördernummer
  1. P50GM082251
  2. CHE0959496
  3. 1 P30 GM110758-01
  4. ANR-10-EQPX-47- 01
  5. 3050 CNRS
  6. 317127
1000 Förderprogramm
  1. -
  2. -
  3. -
  4. -
  5. -
  6. People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme FP7/2007-2013/ under REA grant agreement
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer National Institute of Health |
    1000 Förderprogramm -
    1000 Fördernummer P50GM082251
  2. 1000 joinedFunding-child
    1000 Förderer NSF |
    1000 Förderprogramm -
    1000 Fördernummer CHE0959496
  3. 1000 joinedFunding-child
    1000 Förderer NIGMS |
    1000 Förderprogramm -
    1000 Fördernummer 1 P30 GM110758-01
  4. 1000 joinedFunding-child
    1000 Förderer EQUIPEX |
    1000 Förderprogramm -
    1000 Fördernummer ANR-10-EQPX-47- 01
  5. 1000 joinedFunding-child
    1000 Förderer IR-RMN-THC FRE |
    1000 Förderprogramm -
    1000 Fördernummer 3050 CNRS
  6. 1000 joinedFunding-child
    1000 Förderer European Union |
    1000 Förderprogramm People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme FP7/2007-2013/ under REA grant agreement
    1000 Fördernummer 317127
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403168.rdf
1000 Erstellt am 2017-06-19T15:43:58.062+0200
1000 Erstellt von 22
1000 beschreibt frl:6403168
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Thu Nov 22 11:07:04 CET 2018
1000 Objekt bearb. Thu Nov 22 11:07:03 CET 2018
1000 Vgl. frl:6403168
1000 Oai Id
  1. oai:frl.publisso.de:frl:6403168 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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