WeightNameValue
1000 Titel
  • AKAP18:PKA-RIIα structure reveals crucial anchor points for recognition of regulatory subunits of PKA
1000 Autor/in
  1. Götz, Frank |
  2. Roske, Yvette |
  3. Schulz, Maike Svenja |
  4. Autenrieth, Karolin |
  5. Bertinetti, Daniela |
  6. Faelber, Katja |
  7. Zühlke, Kerstin |
  8. Kreuchwig, Annika |
  9. Kennedy, Eileen J. |
  10. Krause, Gerd |
  11. Daumke, Oliver |
  12. Herberg, Friedrich W. |
  13. Heinemann, Udo |
  14. Klussmann, Enno |
1000 Erscheinungsjahr 2016
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-06-28
1000 Erschienen in
1000 Quellenangabe
  • 473(13): 1881-1894
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964276/ |
  • http://doi.org/10.1042/BCJ20160242 |
1000 Ergänzendes Material
  • http://www.biochemj.org/content/473/13/1881.supplemental |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • A-kinase anchoring proteins (AKAPs) interact with the dimerization/docking (D/D) domains of regulatory subunits of the ubiquitous protein kinase A (PKA). AKAPs tether PKA to defined cellular compartments establishing distinct pools to increase the specificity of PKA signalling. Here, we elucidated the structure of an extended PKA-binding domain of AKAP18β bound to the D/D domain of the regulatory RIIα subunits of PKA. We identified three hydrophilic anchor points in AKAP18β outside the core PKA-binding domain, which mediate contacts with the D/D domain. Such anchor points are conserved within AKAPs that bind regulatory RII subunits of PKA. We derived a different set of anchor points in AKAPs binding regulatory RI subunits of PKA. In vitro and cell-based experiments confirm the relevance of these sites for the interaction of RII subunits with AKAP18 and of RI subunits with the RI-specific smAKAP. Thus we report a novel mechanism governing interactions of AKAPs with PKA. The sequence specificity of each AKAP around the anchor points and the requirement of these points for the tight binding of PKA allow the development of selective inhibitors to unequivocally ascribe cellular functions to the AKAP18-PKA and other AKAP-PKA interactions.
1000 Sacherschließung
lokal D/D domain
lokal PKA-binding domain
lokal compartmentalized cAMP signalling
lokal protein–protein interaction
lokal A-kinase anchoring protein
lokal protein kinase A
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/R8O2dHosIEZyYW5r|https://frl.publisso.de/adhoc/creator/Um9za2UsIFl2ZXR0ZQ==|https://frl.publisso.de/adhoc/creator/U2NodWx6LCBNYWlrZSBTdmVuamE=|https://frl.publisso.de/adhoc/creator/QXV0ZW5yaWV0aCwgS2Fyb2xpbg==|https://frl.publisso.de/adhoc/creator/QmVydGluZXR0aSwgRGFuaWVsYQ==|https://frl.publisso.de/adhoc/creator/RmFlbGJlciwgS2F0amE=|https://frl.publisso.de/adhoc/creator/WsO8aGxrZSwgS2Vyc3Rpbg==|https://frl.publisso.de/adhoc/creator/S3JldWNod2lnLCBBbm5pa2E=|https://frl.publisso.de/adhoc/creator/S2VubmVkeSwgRWlsZWVuIEou|https://frl.publisso.de/adhoc/creator/S3JhdXNlLCBHZXJk|https://frl.publisso.de/adhoc/creator/RGF1bWtlLCBPbGl2ZXI=|https://frl.publisso.de/adhoc/creator/SGVyYmVyZywgRnJpZWRyaWNoIFcu|https://frl.publisso.de/adhoc/creator/SGVpbmVtYW5uLCBVZG8=|https://frl.publisso.de/adhoc/creator/S2x1c3NtYW5uLCBFbm5v
1000 Label
1000 Förderer
  1. Else Kröner–Fresenius–Stiftung |
  2. German–Israeli Foundation |
  3. Deutsche Forschungsgemeinschaft (DFG) |
  4. German Centre for Cardiovascular Research |
1000 Fördernummer
  1. 2013_A145
  2. I-1210-286.13/2012
  3. KL1415/4-2; 1818/6
  4. DZHK 81×210012
1000 Förderprogramm
  1. -
  2. -
  3. -
  4. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Else Kröner–Fresenius–Stiftung |
    1000 Förderprogramm -
    1000 Fördernummer 2013_A145
  2. 1000 joinedFunding-child
    1000 Förderer German–Israeli Foundation |
    1000 Förderprogramm -
    1000 Fördernummer I-1210-286.13/2012
  3. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft (DFG) |
    1000 Förderprogramm -
    1000 Fördernummer KL1415/4-2; 1818/6
  4. 1000 joinedFunding-child
    1000 Förderer German Centre for Cardiovascular Research |
    1000 Förderprogramm -
    1000 Fördernummer DZHK 81×210012
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403525.rdf
1000 Erstellt am 2017-07-18T14:06:00.707+0200
1000 Erstellt von 25
1000 beschreibt frl:6403525
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Fri Jan 31 02:00:53 CET 2020
1000 Objekt bearb. Thu Sep 27 13:41:52 CEST 2018
1000 Vgl. frl:6403525
1000 Oai Id
  1. oai:frl.publisso.de:frl:6403525 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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