fphys-08-00380.pdf 1,48MB
1000 Titel
  • Neuronal Chemosensation and Osmotic Stress Response Converge in the Regulation of aqp-8 in C. elegans
1000 Autor/in
  1. Igual Gil, Carla |
  2. Jarius, Mirko |
  3. von Kries, Jens P. |
  4. Rohlfing, Anne-Katrin |
1000 Erscheinungsjahr 2017
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-06-09
1000 Erschienen in
1000 Quellenangabe
  • 8: 380
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2017
1000 Lizenz
1000 Verlagsversion
  • |
  • |
1000 Ergänzendes Material
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Aquaporins occupy an essential role in sustaining the salt/water balance in various cells types and tissues. Here, we present new insights into aqp-8 expression and regulation in Caenorhabditis elegans. We show, that upon exposure to osmotic stress, aqp-8 exhibits a distinct expression pattern within the excretory cell compared to other C. elegans aquaporins expressed. This expression is correlated to the osmolarity of the surrounding medium and can be activated physiologically by osmotic stress or genetically in mutants with constitutively active osmotic stress response. In addition, we found aqp-8 expression to be constitutively active in the TRPV channel mutant osm-9(ok1677). In a genome-wide RNAi screen we identified additional regulators of aqp-8. Many of these regulators are connected to chemosensation by the amphid neurons, e.g., odr-10 and gpa-6, and act as suppressors of aqp-8 expression. We postulate from our results, that aqp-8 plays an important role in sustaining the salt/water balance during a secondary response to hyper-osmotic stress. Upon its activation aqp-8 promotes vesicle docking to the lumen of the excretory cell and thereby enhances the ability to secrete water and transport osmotic active substances or waste products caused by protein damage. In summary, aqp-8 expression and function is tightly regulated by a network consisting of the osmotic stress response, neuronal chemosensation as well as the response to protein damage. These new insights in maintaining the salt/water balance in C. elegans will help to reveal the complex homeostasis network preserved throughout species.
1000 Sacherschließung
lokal chemosensation
lokal C. elegans
lokal osmoregulation
lokal aquaporin
lokal osmotic stress
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
1000 Label
1000 Förderer
  1. NIH Office of Research Infrastructure Programs |
1000 Fördernummer
  1. P40 OD010440
1000 Förderprogramm
  1. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer NIH Office of Research Infrastructure Programs |
    1000 Förderprogramm -
    1000 Fördernummer P40 OD010440
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403535.rdf
1000 Erstellt am 2017-07-19T13:37:06.877+0200
1000 Erstellt von 25
1000 beschreibt frl:6403535
1000 Bearbeitet von 288
1000 Zuletzt bearbeitet Wed Mar 31 09:10:28 CEST 2021
1000 Objekt bearb. Wed Mar 31 09:10:28 CEST 2021
1000 Vgl. frl:6403535
1000 Oai Id
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