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1000 Titel
  • Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq
1000 Autor/in
  1. Marthandan, S. |
  2. Priebe, S. |
  3. Schaer, J. |
  4. Kaether, C. |
  5. Guthke, R. |
  6. Diekmann, S. |
  7. Hemmerich, P. |
  8. Baumgart, Mario |
  9. Groth, Marco |
  10. Cellerino, Alessandro |
  11. Platzer, Matthias |
1000 Erscheinungsjahr 2016
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-05-03
1000 Erschienen in
1000 Quellenangabe
  • 11(5): e0154531
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1371/journal.pone.0154531 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4854426/ |
1000 Ergänzendes Material
  • http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0154531#sec037 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Cellular senescence correlates with changes in the transcriptome. To obtain a complete view on senescence-associated transcription networks and pathways, we assessed by deep RNA sequencing the transcriptomes of five of the most commonly used laboratory strains of human fibroblasts during their transition into senescence. In a number of cases, we verified the RNA-seq data by real-time PCR. By determining cellular protein levels we observed that the age-related expression of most but not all genes is regulated at the transcriptional level. We found that 78% of the age-affected differentially expressed genes were commonly regulated in the same direction (either up- or down-regulated) in all five fibroblast strains, indicating a strong conservation of age-associated changes in the transcriptome. KEGG pathway analyses confirmed up-regulation of the senescence-associated secretory phenotype and down-regulation of DNA synthesis/repair and most cell cycle pathways common in all five cell strains. Newly identified senescence-induced pathways include up-regulation of endocytotic/phagocytic pathways and down-regulation of the mRNA metabolism and the mRNA splicing pathways. Our results provide an unprecedented comprehensive and deep view into the individual and common transcriptome and pathway changes during the transition into of senescence of five human fibroblast cell strains.
1000 Sacherschließung
lokal Senescence
lokal Microarrays
lokal Gene regulation
lokal Fibroblasts
lokal Lung development
lokal Gene expression
lokal Recombinant proteins
lokal Cell cycle and cell division
1000 Fachgruppe
  1. Biologie |
  2. Medizin |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/TWFydGhhbmRhbiwgUy4=|https://frl.publisso.de/adhoc/creator/UHJpZWJlLCBTLg==|https://frl.publisso.de/adhoc/creator/U2NoYWVyLCBKLg==|https://frl.publisso.de/adhoc/creator/S2FldGhlciwgQy4=|https://frl.publisso.de/adhoc/creator/R3V0aGtlLCBSLg==|https://frl.publisso.de/adhoc/creator/RGlla21hbm4sIFMu|https://frl.publisso.de/adhoc/creator/SGVtbWVyaWNoLCBQLg==|http://orcid.org/0000-0001-8172-1595|http://orcid.org/0000-0002-9199-8990|http://orcid.org/0000-0003-3834-0097|http://orcid.org/0000-0003-0596-8582
1000 Förderer
  1. German Ministry for Education and Research (Bundesministerium fur Bildung und Forschung - BMBF) |
1000 Fördernummer
  1. 0315581
1000 Förderprogramm
  1. Research program of the Jena Centre for Systems Biology of Ageing
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Ministry for Education and Research (Bundesministerium fur Bildung und Forschung - BMBF) |
    1000 Förderprogramm Research program of the Jena Centre for Systems Biology of Ageing
    1000 Fördernummer 0315581
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6404004.rdf
1000 Erstellt am 2017-08-18T16:20:55.328+0200
1000 Erstellt von 218
1000 beschreibt frl:6404004
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Mon May 07 12:17:55 CEST 2018
1000 Objekt bearb. Mon May 07 12:17:55 CEST 2018
1000 Vgl. frl:6404004
1000 Oai Id
  1. oai:frl.publisso.de:frl:6404004 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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