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WeightNameValue
1000 Titel
  • Advanced Running Performance by Genetic Predisposition in Male Dummerstorf Marathon Mice (DUhTP) Reveals Higher Sterol Regulatory Element-Binding Protein (SREBP) Related mRNA Expression in the Liver and Higher Serum Levels of Progesterone
1000 Autor/in
  1. Ohde, Daniela |
  2. Moeller, Mark |
  3. Brenmoehl, Julia |
  4. Walz, Christina |
  5. Ponsuksili, Siriluck |
  6. Schwerin, Manfred |
  7. Fuellen, Georg |
  8. Hoeflich, Andreas |
1000 Erscheinungsjahr 2016
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-01-22
1000 Erschienen in
1000 Quellenangabe
  • 11(1):e0146748
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • https://dx.doi.org/10.1371/journal.pone.0146748 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4723140/ |
1000 Ergänzendes Material
  • http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0146748#sec023 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Long-term-selected DUhTP mice represent a non-inbred model for inborn physical high-performance without previous training. Abundance of hepatic mRNA in 70-day male DUhTP and control mice was analyzed using the Affymetrix mouse array 430A 2.0. Differential expression analysis with PLIER corrected data was performed using AltAnalyze. Searching for over-representation in biochemical pathways revealed cholesterol metabolism being most prominently affected in DUhTP compared to unselected control mice. Furthermore, pathway analysis by AltAnalyze plus PathVisio indicated significant induction of glycolysis, fatty acid synthesis and cholesterol biosynthesis in the liver of DUhTP mice versus unselected control mice. In contrast, gluconeogenesis was partially inactivated as judged from the analysis of hepatic mRNA transcript abundance in DUhTP mice. Analysis of mRNA transcripts related to steroid hormone metabolism inferred elevated synthesis of progesterone and reduced levels of sex steroids. Abundance of steroid delta isomerase-5 mRNA (Hsd3b5, FC 4.97) was increased and steroid 17-alpha-monooxygenase mRNA (Cyp17a1, FC -11.6) was massively diminished in the liver of DUhTP mice. Assessment of steroid profiles by LC-MS revealed increased levels of progesterone and decreased levels of sex steroids in serum from DUhTP mice versus controls. Analysis of hepatic mRNA transcript abundance indicates that sterol regulatory element-binding protein-1 (SREBP-1) may play a major role in metabolic pathway activation in the marathon mouse model DUhTP. Thus, results from bioinformatics modeling of hepatic mRNA transcript abundance correlated with direct steroid analysis by mass spectrometry and further indicated functions of SREBP-1 and steroid hormones for endurance performance in DUhTP mice.
1000 Sacherschließung
lokal Messenger RNA
lokal Microarrays
lokal Mouse models
lokal Biosynthesis
lokal Gene expression
lokal Fatty acids
lokal Cholesterol
lokal Steroids
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/T2hkZSwgRGFuaWVsYQ==|https://frl.publisso.de/adhoc/creator/TW9lbGxlciwgTWFyaw==|https://frl.publisso.de/adhoc/creator/QnJlbm1vZWhsLCBKdWxpYQ==|https://frl.publisso.de/adhoc/creator/V2FseiwgQ2hyaXN0aW5h|https://frl.publisso.de/adhoc/creator/UG9uc3Vrc2lsaSwgU2lyaWx1Y2s=|https://frl.publisso.de/adhoc/creator/U2Nod2VyaW4sIE1hbmZyZWQ=|https://frl.publisso.de/adhoc/creator/RnVlbGxlbiwgR2Vvcmc=|http://orcid.org/0000-0003-2018-2836
1000 Förderer
  1. H. Wilhelm Schaumann Stiftung |
  2. Bundesministerium für Bildung und Forschung (BMBF) |
  3. Deutsche Forschungsgemeinschaft (DFG) |
  4. European Commission |
1000 Fördernummer
  1. -
  2. FKZ 0315892; FKZ 0315892A
  3. HO 2003/6-1
  4. -
  5. 633589
1000 Förderprogramm
  1. stipend
  2. ROSAge project
  3. -
  4. Horizon 2020
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer H. Wilhelm Schaumann Stiftung |
    1000 Förderprogramm stipend
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Bundesministerium für Bildung und Forschung (BMBF) |
    1000 Förderprogramm ROSAge project
    1000 Fördernummer FKZ 0315892; FKZ 0315892A
  3. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft (DFG) |
    1000 Förderprogramm -
    1000 Fördernummer HO 2003/6-1
  4. 1000 joinedFunding-child
    1000 Förderer European Commission |
    1000 Förderprogramm Horizon 2020
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6404139.rdf
1000 Erstellt am 2017-08-25T11:36:13.195+0200
1000 Erstellt von 122
1000 beschreibt frl:6404139
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Mon Apr 30 13:24:14 CEST 2018
1000 Objekt bearb. Mon Apr 30 13:24:13 CEST 2018
1000 Vgl. frl:6404139
1000 Oai Id
  1. oai:frl.publisso.de:frl:6404139 |
1000 Sichtbarkeit Metadaten public
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