WeightNameValue
1000 Titel
  • WW domain sequence activity relationships identified using ligand recognition propensities of 42 WW domains
1000 Autor/in
  1. Otte, Livia |
  2. Wiedemann, Urs |
  3. Schlegel, Brigitte |
  4. Pires, José Ricardo |
  5. Beyermann, Michae |
  6. Krause, Gerd |
  7. Volkmer-Engert, Rudolf |
  8. Schneider-Mergener, Jens |
  9. Oschkinat, Hartmut |
  10. Schmieder, Peter |
1000 Erscheinungsjahr 2009
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2009-01-01
1000 Erschienen in
1000 Quellenangabe
  • 12(3): 491-500
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2312455/ |
  • https://dx.doi.org/10.1110/ps.0233203 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • WW domains mediate protein–protein interactions in a number of different cellular functions by recognizing proline-containing peptide sequences. We determined peptide recognition propensities for 42 WW domains using NMR spectroscopy and peptide library screens. As potential ligands, we studied both model peptides and peptides based on naturally occurring sequences, including phosphorylated residues. Thirty-two WW domains were classified into six groups according to detected ligand recognition preferences for binding the motifs PPx(Y/poY), (p/φ)P(p,g)PPpR, (p/φ)PPRgpPp, PPLPp, (p/ξ)PPPPP, and (poS/poT)P (motifs according to modified Seefeld Convention 2001). In addition to these distinct binding motifs, group-specific WW domain consensus sequences were identified. For PPxY-recognizing domains, phospho-tyrosine binding was also observed. Based on the sequences of the PPx(Y/poY)-specific group, a profile hidden Markov model was calculated and used to predict PPx(Y/poY)-recognition activity for WW domains, which were not assayed. PPx(Y/poY)-binding was found to be a common property of NEDD4-like ubiquitin ligases.
1000 Sacherschließung
lokal peptide libraries
lokal protein interaction modules
lokal WW domain
lokal Protein—protein interaction
lokal ligand screen
lokal sequence—activity relationships
lokal protein—protein interaction prediction
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/T3R0ZSwgTGl2aWE=|https://frl.publisso.de/adhoc/creator/V2llZGVtYW5uLCBVcnM=|https://frl.publisso.de/adhoc/creator/U2NobGVnZWwsIEJyaWdpdHRl|https://frl.publisso.de/adhoc/creator/UGlyZXMsIEpvc8OpIFJpY2FyZG8=|https://frl.publisso.de/adhoc/creator/QmV5ZXJtYW5uLCBNaWNoYWU=|https://frl.publisso.de/adhoc/creator/S3JhdXNlLCBHZXJk|https://frl.publisso.de/adhoc/creator/Vm9sa21lci1FbmdlcnQsIFJ1ZG9sZg==|https://frl.publisso.de/adhoc/creator/U2NobmVpZGVyLU1lcmdlbmVyLCBKZW5z|https://frl.publisso.de/adhoc/creator/T3NjaGtpbmF0LCBIYXJ0bXV0|http://orcid.org/0000-0001-9968-9327
1000 Label
1000 Förderer
  1. Berlin Structure Factory Project |
  2. Deutsche Forschungsgemeinschaft (DFG) |
1000 Fördernummer
  1. -
  2. -
1000 Förderprogramm
  1. -
  2. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Berlin Structure Factory Project |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft (DFG) |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6404466.rdf
1000 Erstellt am 2017-09-15T11:20:08.323+0200
1000 Erstellt von 122
1000 beschreibt frl:6404466
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Thu Jan 30 18:12:58 CET 2020
1000 Objekt bearb. Wed Aug 01 12:24:27 CEST 2018
1000 Vgl. frl:6404466
1000 Oai Id
  1. oai:frl.publisso.de:frl:6404466 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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