WeightNameValue
1000 Titel
  • A small region in phosducin inhibits G‐protein βγ‐subunit function
1000 Autor/in
  1. Blüml, Klaus |
  2. Schnepp, Werner |
  3. Schröder, Stefan |
  4. Beyermann, Michael |
  5. Macias, Maria |
  6. Oschkinat, Hartmut |
  7. Lohse, Martin J. |
1000 Erscheinungsjahr 1997
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 1997-08-15
1000 Erschienen in
1000 Quellenangabe
  • 16(16): 4908-4915
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1170126/ |
  • http://dx.doi.org/10.1093/emboj/16.16.4908 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • G-protein betagamma-subunits (G(betagamma)) are active transmembrane signalling components. Their function recently has been observed to be regulated by the cytosolic protein phosducin. We show here that a small fragment (amino acids 215-232) contained in the C-terminus of phosducin is sufficient for high-affinity interactions with G(betagamma). Corresponding peptides not only disrupt G(betagamma)-G(alpha) interactions, as defined by G(betagamma)-stimulated GTPase activity of alpha(o), but also other G(betagamma)-mediated functions. The NMR structure of a peptide encompassing this region shows a loop exposing the side chains of Glu223 and Tyr224, and peptides with a substitution of either of these amino acids show a complete loss of activity towards G(o). Mutation of this Tyr224 to Ala in full-length phosducin reduced the functional activity of phosducin to that of phosducin's isolated N-terminus, indicating the importance of this residue within the short, structurally defined C-terminal segment. This small peptide derived from phosducin, may represent a model of a G(betagamma) inhibitor, and illustrates the potential of small compounds to affect G(betagamma) functions.
1000 Sacherschließung
lokal B-adrenergic Receptor Kinase
lokal Protein NMR
lokal Adenylyl Cyclase
lokal G-proteins
lokal Phosducin
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/QmzDvG1sLCBLbGF1cw==|https://frl.publisso.de/adhoc/creator/U2NobmVwcCwgV2VybmVy|https://frl.publisso.de/adhoc/creator/U2NocsO2ZGVyLCBTdGVmYW4=|https://frl.publisso.de/adhoc/creator/QmV5ZXJtYW5uLCBNaWNoYWVs|https://frl.publisso.de/adhoc/creator/TWFjaWFzLCBNYXJpYQ==|https://frl.publisso.de/adhoc/creator/T3NjaGtpbmF0LCBIYXJ0bXV0|https://frl.publisso.de/adhoc/creator/TG9oc2UsIE1hcnRpbiBKLg==
1000 Förderer
  1. Deutsche Forschungsgemeinschaft (DFG) |
  2. Fonds der Chemischen Industrie |
1000 Fördernummer
  1. -
  2. -
1000 Förderprogramm
  1. SFB 176; SPP GTPasen
  2. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft (DFG) |
    1000 Förderprogramm SFB 176; SPP GTPasen
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Fonds der Chemischen Industrie |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6404500.rdf
1000 Erstellt am 2017-09-20T09:26:16.754+0200
1000 Erstellt von 122
1000 beschreibt frl:6404500
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Wed Aug 01 11:51:09 CEST 2018
1000 Objekt bearb. Wed Aug 01 11:51:08 CEST 2018
1000 Vgl. frl:6404500
1000 Oai Id
  1. oai:frl.publisso.de:frl:6404500 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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