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1000 Titel
  • Determination of nasal and oropharyngeal microbiomes in a multicenter population-based study – findings from Pretest 1 of the German National Cohort
1000 Autor/in
  1. Akmatov, Manas K |
  2. Koch, Nadine |
  3. Vital, Marius |
  4. Flesch-Janys, Dieter |
  5. Fricke, Julia |
  6. Gatzemeier, Anja |
  7. Greiser, Halina |
  8. Günther, Kathrin |
  9. Illig, Thomas |
  10. Kaaks, Rudolf |
  11. Krone, Bastian |
  12. Kühn, Andrea |
  13. Linseisen, Jakob |
  14. Meisinger, Christine |
  15. Michels, Karin |
  16. Moebus, Susanne |
  17. Nieters, Alexandra |
  18. Obi, Nadia |
  19. Schultze, Anja |
  20. Six-Merker, Julia |
  21. Pieper, Dietmar H |
  22. Pessler, Frank |
  23. Ahrens, Wolfgang |
1000 Erscheinungsjahr 2017
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-05-12
1000 Erschienen in
1000 Quellenangabe
  • 7:1855
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2017
1000 Lizenz
1000 Verlagsversion
  • http://dx.doi.org/10.1038/s41598-017-01212-6 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431815/ |
1000 Ergänzendes Material
  • https://www.nature.com/articles/s41598-017-01212-6#supplementary-information |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • We examined acceptability, preference and feasibility of collecting nasal and oropharyngeal swabs, followed by microbiome analysis, in a population-based study with 524 participants. Anterior nasal and oropharyngeal swabs were collected by certified personnel. In addition, participants self-collected nasal swabs at home four weeks later. Four swab types were compared regarding (1) participants’ satisfaction and acceptance and (2) detection of microbial community structures based on deep sequencing of the 16 S rRNA gene V1–V2 variable regions. All swabbing methods were highly accepted. Microbial community structure analysis revealed 846 phylotypes, 46 of which were unique to oropharynx and 164 unique to nares. The calcium alginate tipped swab was found unsuitable for microbiome determinations. Among the remaining three swab types, there were no differences in oropharyngeal microbiomes detected and only marginal differences in nasal microbiomes. Microbial community structures did not differ between staff-collected and self-collected nasal swabs. These results suggest (1) that nasal and oropharyngeal swabbing are highly feasible methods for human population-based studies that include the characterization of microbial community structures in these important ecological niches, and (2) that self-collection of nasal swabs at home can be used to reduce cost and resources needed, particularly when serial measurements are to be taken.
1000 Sacherschließung
lokal epidemiology
lokal bacterial infection
lokal microbiome
1000 Fachgruppe
  1. Medizin |
  2. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/QWttYXRvdiwgTWFuYXMgSw==|https://frl.publisso.de/adhoc/creator/S29jaCwgTmFkaW5l|https://frl.publisso.de/adhoc/creator/Vml0YWwsIE1hcml1cw==|https://frl.publisso.de/adhoc/creator/Rmxlc2NoLUphbnlzLCBEaWV0ZXI=|https://frl.publisso.de/adhoc/creator/RnJpY2tlLCBKdWxpYQ==|https://frl.publisso.de/adhoc/creator/R2F0emVtZWllciwgQW5qYQ==|https://frl.publisso.de/adhoc/creator/R3JlaXNlciwgSGFsaW5h|https://frl.publisso.de/adhoc/creator/R8O8bnRoZXIsIEthdGhyaW4=|https://frl.publisso.de/adhoc/creator/SWxsaWcsIFRob21hcw==|https://frl.publisso.de/adhoc/creator/S2Fha3MsIFJ1ZG9sZg==|https://frl.publisso.de/adhoc/creator/S3JvbmUsIEJhc3RpYW4=|https://frl.publisso.de/adhoc/creator/S8O8aG4sIEFuZHJlYQ==|https://frl.publisso.de/adhoc/creator/TGluc2Vpc2VuLCBKYWtvYg==|https://frl.publisso.de/adhoc/creator/TWVpc2luZ2VyLCBDaHJpc3RpbmU=|https://frl.publisso.de/adhoc/creator/TWljaGVscywgS2FyaW4=|https://frl.publisso.de/adhoc/creator/TW9lYnVzLCBTdXNhbm5l|https://frl.publisso.de/adhoc/creator/TmlldGVycywgQWxleGFuZHJh|https://frl.publisso.de/adhoc/creator/T2JpLCBOYWRpYQ==|https://frl.publisso.de/adhoc/creator/U2NodWx0emUsIEFuamE=|https://frl.publisso.de/adhoc/creator/U2l4LU1lcmtlciwgSnVsaWE=|https://frl.publisso.de/adhoc/creator/UGllcGVyLCBEaWV0bWFyIEg=|https://frl.publisso.de/adhoc/creator/UGVzc2xlciwgRnJhbms=|http://orcid.org/0000-0003-3777-570X
1000 Förderer
  1. Federal Ministry of Education and Research (BMBF) |
  2. European Union |
  3. iMed, the Helmholtz Associations’s Initiative on Personalized Medicine |
1000 Fördernummer
  1. 01EO1303; 01ER1001B; 01ER1001G
  2. FP7/2007-2013
  3. -
1000 Förderprogramm
  1. -
  2. Seventh Framework Programme
  3. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Federal Ministry of Education and Research (BMBF) |
    1000 Förderprogramm -
    1000 Fördernummer 01EO1303; 01ER1001B; 01ER1001G
  2. 1000 joinedFunding-child
    1000 Förderer European Union |
    1000 Förderprogramm Seventh Framework Programme
    1000 Fördernummer FP7/2007-2013
  3. 1000 joinedFunding-child
    1000 Förderer iMed, the Helmholtz Associations’s Initiative on Personalized Medicine |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6404553.rdf
1000 Erstellt am 2017-09-21T14:55:01.472+0200
1000 Erstellt von 122
1000 beschreibt frl:6404553
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Mon May 07 12:49:01 CEST 2018
1000 Objekt bearb. Mon May 07 12:49:01 CEST 2018
1000 Vgl. frl:6404553
1000 Oai Id
  1. oai:frl.publisso.de:frl:6404553 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
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