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1000 Titel
  • Signal Peptide Cleavage from GP5 of PRRSV: A Minor Fraction of Molecules Retains the Decoy Epitope, a Presumed Molecular Cause for Viral Persistence
1000 Autor/in
  1. Thaa, Bastian |
  2. Sinhadri, Balaji Chandrasekhar |
  3. Tielesch, Claudia |
  4. Krause, Eberhard |
  5. Veit, Michael |
1000 Erscheinungsjahr 2013
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2013-06-06
1000 Erschienen in
1000 Quellenangabe
  • 8(6): e65548
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2013
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1371/journal.pone.0065548 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3675037/ |
1000 Ergänzendes Material
  • http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0065548#s5 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Porcine reproductive and respiratory syndrome virus (PRRSV) is the major pathogen in the pig industry. Variability of the antigens and persistence are the biggest challenges for successful control and elimination of the disease. GP5, the major glycoprotein of PRRSV, is considered an important target of neutralizing antibodies, which however appear only late in infection. This was attributed to the presence of a “decoy epitope” located near a hypervariable region of GP5. This region also harbors the predicted signal peptide cleavage sites and (dependent on the virus strain) a variable number of potential N-glycosylation sites. Molecular processing of GP5 has not been addressed experimentally so far: whether and where the signal peptide is cleaved and (as a consequence) whether the “decoy epitope” is present in virus particles. We show that the signal peptide of GP5 from the American type 2 reference strain VR-2332 is cleaved, both during in vitro translation in the presence of microsomes and in transfected cells. This was found to be independent of neighboring glycosylation sites and occurred in a variety of porcine cells for GP5 sequences derived from various type 2 strains. The exact signal peptide cleavage site was elucidated by mass spectrometry of virus-derived and recombinant GP5. The results revealed that the signal peptide of GP5 is cleaved at two sites. As a result, a mixture of GP5 proteins exists in virus particles, some of which still contain the “decoy epitope” sequence. Heterogeneity was also observed for the use of glycosylation sites in the hypervariable region. Lastly, GP5 mutants were engineered where one of the signal peptide cleavage sites was blocked. Wildtype GP5 exhibited exactly the same SDS-PAGE mobility as the mutant that is cleavable at site 2 only. This indicates that the overwhelming majority of all GP5 molecules does not contain the “decoy epitope”.
1000 Sacherschließung
lokal Carbohydrates
lokal Signal peptides
lokal Glycosylation
lokal Respiratory infections
lokal Recombinant proteins
lokal Microsomes
lokal Swine
lokal Antibodies
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/VGhhYSwgQmFzdGlhbg==|https://frl.publisso.de/adhoc/creator/U2luaGFkcmksIEJhbGFqaSBDaGFuZHJhc2VraGFy|https://frl.publisso.de/adhoc/creator/VGllbGVzY2gsIENsYXVkaWE=|https://frl.publisso.de/adhoc/creator/S3JhdXNlLCBFYmVyaGFyZA==|https://frl.publisso.de/adhoc/creator/VmVpdCwgTWljaGFlbA==
1000 Label
1000 Förderer
  1. German Research Foundation (DFG) |
  2. Collaborative Research Center 740 |
  3. European Commission |
  4. Marie Curie Initial Trainig Network |
  5. Boehringer Ingelheim Vetmedica GmbH |
1000 Fördernummer
  1. Ve 141/11-1
  2. -
  3. -
  4. -
  5. -
1000 Förderprogramm
  1. -
  2. -
  3. EU FP7
  4. “Molecular Mechanism of cell entry of enveloped viruses”
  5. PRRSV Research Award
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Research Foundation (DFG) |
    1000 Förderprogramm -
    1000 Fördernummer Ve 141/11-1
  2. 1000 joinedFunding-child
    1000 Förderer Collaborative Research Center 740 |
    1000 Förderprogramm -
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer European Commission |
    1000 Förderprogramm EU FP7
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer Marie Curie Initial Trainig Network |
    1000 Förderprogramm “Molecular Mechanism of cell entry of enveloped viruses”
    1000 Fördernummer -
  5. 1000 joinedFunding-child
    1000 Förderer Boehringer Ingelheim Vetmedica GmbH |
    1000 Förderprogramm PRRSV Research Award
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6404571.rdf
1000 Erstellt am 2017-09-22T09:46:22.259+0200
1000 Erstellt von 218
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1000 Bearbeitet von 288
1000 Zuletzt bearbeitet Thu Aug 18 07:53:03 CEST 2022
1000 Objekt bearb. Thu Mar 04 12:15:40 CET 2021
1000 Vgl. frl:6404571
1000 Oai Id
  1. oai:frl.publisso.de:frl:6404571 |
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