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1000 Titel
  • A complex of Neuroplastin and Plasma Membrane Ca2+ ATPase controls T cell activation
1000 Autor/in
  1. Korthals, Mark |
  2. Langnaese, Kristina |
  3. Smalla, Dr. Karl-Heinz |
  4. Kähne, Thilo |
  5. Herrera-Molina, Rodrigo |
  6. Handschuh, Juliane |
  7. Lehmann, Anne-Christin |
  8. Mamula, Dejan |
  9. Naumann, Michael |
  10. Seidenbecher, Constanze |
  11. Zuschratter, Werner |
  12. Tedford, Kerry |
  13. Gundelfinger, Eckart |
  14. Montag, Dirk |
  15. Fischer, Klaus-Dieter |
  16. Thomas, Ulrich |
1000 Erscheinungsjahr 2017
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-08-21
1000 Erschienen in
1000 Quellenangabe
  • 7:8358
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2017
1000 Lizenz
1000 Verlagsversion
  • http://dx.doi.org/10.1038/s41598-017-08519-4 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566957/ |
1000 Ergänzendes Material
  • https://www.nature.com/articles/s41598-017-08519-4#supplementary-information |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The outcome of T cell activation is determined by mechanisms that balance Ca2+ influx and clearance. Here we report that murine CD4 T cells lacking Neuroplastin (Nptn -/-), an immunoglobulin superfamily protein, display elevated cytosolic Ca2+ and impaired post-stimulation Ca2+ clearance, along with increased nuclear levels of NFAT transcription factor and enhanced T cell receptor-induced cytokine production. On the molecular level, we identified plasma membrane Ca2+ ATPases (PMCAs) as the main interaction partners of Neuroplastin. PMCA levels were reduced by over 70% in Nptn -/- T cells, suggesting an explanation for altered Ca2+ handling. Supporting this, Ca2+ extrusion was impaired while Ca2+ levels in internal stores were increased. T cells heterozygous for PMCA1 mimicked the phenotype of Nptn -/- T cells. Consistent with sustained Ca2+ levels, differentiation of Nptn -/- T helper cells was biased towards the Th1 versus Th2 subset. Our study thus establishes Neuroplastin-PMCA modules as important regulators of T cell activation.
1000 Sacherschließung
lokal Signal transduction
lokal Calcium signalling
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/S29ydGhhbHMsIE1hcms=|https://frl.publisso.de/adhoc/creator/TGFuZ25hZXNlLCBLcmlzdGluYQ==|https://orcid.org/0000-0002-0269-0311|https://frl.publisso.de/adhoc/creator/S8OkaG5lLCBUaGlsbw==|https://frl.publisso.de/adhoc/creator/SGVycmVyYS1Nb2xpbmEsIFJvZHJpZ28=|https://frl.publisso.de/adhoc/creator/SGFuZHNjaHVoLCBKdWxpYW5l|https://frl.publisso.de/adhoc/creator/TGVobWFubiwgQW5uZS1DaHJpc3Rpbg==|https://frl.publisso.de/adhoc/creator/TWFtdWxhLCBEZWphbg==|https://frl.publisso.de/adhoc/creator/TmF1bWFubiwgTWljaGFlbA==|https://frl.publisso.de/adhoc/creator/U2VpZGVuYmVjaGVyLCBDb25zdGFuemU=|https://frl.publisso.de/adhoc/creator/WnVzY2hyYXR0ZXIsIFdlcm5lcg==|https://frl.publisso.de/adhoc/creator/VGVkZm9yZCwgS2Vycnk=|http://orcid.org/0000-0001-9377-7414|http://orcid.org/0000-0002-4964-1330|https://frl.publisso.de/adhoc/creator/RmlzY2hlciwgS2xhdXMtRGlldGVy|https://frl.publisso.de/adhoc/creator/VGhvbWFzLCBVbHJpY2g=
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft (DFG) |
  2. BMBF |
  3. Ministry for Economics, Science, and Digitization of the State Saxony-Anhalt |
  4. European Fonds for Social and Regional Development (EFRE, ESF) |
1000 Fördernummer
  1. SFB854-B08; SFB854-A04; SFB854-Z; SFB426
  2. CHL15WTZ- IB-049
  3. -
  4. -
1000 Förderprogramm
  1. -
  2. -
  3. ABINEP graduate school
  4. ABINEP graduate school
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft (DFG) |
    1000 Förderprogramm -
    1000 Fördernummer SFB854-B08; SFB854-A04; SFB854-Z; SFB426
  2. 1000 joinedFunding-child
    1000 Förderer BMBF |
    1000 Förderprogramm -
    1000 Fördernummer CHL15WTZ- IB-049
  3. 1000 joinedFunding-child
    1000 Förderer Ministry for Economics, Science, and Digitization of the State Saxony-Anhalt |
    1000 Förderprogramm ABINEP graduate school
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer European Fonds for Social and Regional Development (EFRE, ESF) |
    1000 Förderprogramm ABINEP graduate school
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6404792.rdf
1000 Erstellt am 2017-10-02T11:52:54.145+0200
1000 Erstellt von 122
1000 beschreibt frl:6404792
1000 Bearbeitet von 288
1000 Zuletzt bearbeitet Thu Apr 08 09:18:15 CEST 2021
1000 Objekt bearb. Thu Apr 08 09:18:15 CEST 2021
1000 Vgl. frl:6404792
1000 Oai Id
  1. oai:frl.publisso.de:frl:6404792 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
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