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10.1371/journal.pone.0102425.PDF 3,73MB
WeightNameValue
1000 Titel
  • Liposome Reconstitution and Modulation of Recombinant Prenylated Human Rac1 by GEFs, GDI1 and Pak1
1000 Autor/in
  1. Zhang, Si-Cai |
  2. Gremer, Lothar |
  3. Heise, Henrike |
  4. Janning, Petra |
  5. Shymanets, Aliaksei |
  6. Cirstea, Ion C. |
  7. Krause, Eberhard |
  8. Nürnberg, Bernd |
  9. Ahmadian, Mohammad Reza |
1000 Erscheinungsjahr 2014
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2014-07-11
1000 Erschienen in
1000 Quellenangabe
  • 9(7):e102425
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2014
1000 Lizenz
1000 Verlagsversion
  • http://dx.doi.org/10.1371/journal.pone.0102425 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094549/ |
1000 Ergänzendes Material
  • http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0102425#s5 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Small Rho GTPases are well known to regulate a variety of cellular processes by acting as molecular switches. The regulatory function of Rho GTPases is critically dependent on their posttranslational modification at the carboxyl terminus by isoprenylation and association with proper cellular membranes. Despite numerous studies, the mechanisms of recycling and functional integration of Rho GTPases at the biological membranes are largely unclear. In this study, prenylated human Rac1, a prominent member of the Rho family, was purified in large amount from baculovirus-infected Spodoptera frugiperda insect cells using a systematic detergent screening. In contrast to non-prenylated human Rac1 purified from Escherichia coli, prenylated Rac1 from insect cells was able to associate with synthetic liposomes and to bind Rho-specific guanine nucleotide dissociation inhibitor 1 (GDI1). Subsequent liposome reconstitution experiments revealed that GDI1 efficiently extracts Rac1 from liposomes preferentially in the inactive GDP-bound state. The extraction was prevented when Rac1 was activated to its GTP-bound state by Rac-specific guanine nucleotide exchange factors (GEFs), such as Vav2, Dbl, Tiam1, P-Rex1 and TrioN, and bound by the downstream effector Pak1. We found that dissociation of Rac1-GDP from its complex with GDI1 strongly correlated with two distinct activities of especially Dbl and Tiam1, including liposome association and the GDP/GTP exchange. Taken together, our results provided first detailed insights into the advantages of the in vitro liposome-based reconstitution system to study both the integration of the signal transducing protein complexes and the mechanisms of regulation and signaling of small GTPases at biological membranes.
1000 Sacherschließung
lokal Guanine nucleotide exchange factors
lokal Insects
lokal Lipids
lokal Cell membranes
lokal Liposomes
lokal Detergents
lokal Guanosine triphosphatase
lokal Sedimentation
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/WmhhbmcsIFNpLUNhaQ==|https://frl.publisso.de/adhoc/creator/R3JlbWVyLCBMb3RoYXI=|https://frl.publisso.de/adhoc/creator/SGVpc2UsIEhlbnJpa2U=|https://frl.publisso.de/adhoc/creator/SmFubmluZywgUGV0cmE=|https://frl.publisso.de/adhoc/creator/U2h5bWFuZXRzLCBBbGlha3NlaQ==|https://frl.publisso.de/adhoc/creator/Q2lyc3RlYSwgSW9uIEMu|https://frl.publisso.de/adhoc/creator/S3JhdXNlLCBFYmVyaGFyZA==|https://frl.publisso.de/adhoc/creator/TsO8cm5iZXJnLCBCZXJuZA==|https://frl.publisso.de/adhoc/creator/QWhtYWRpYW4sIE1vaGFtbWFkIFJlemE=
1000 Label
1000 Förderer
  1. German Research Foundation (Deutsche Forschungsgemeinschaft or DFG) |
  2. Ministry of Innovation, Science and Research of the State North Rhine-Westphalia |
  3. Heinrich-Heine-University of Düsseldorf |
  4. Entrepreneur Foundation at the Heinrich-Heine-University of Düsseldorf |
1000 Fördernummer
  1. SFB 974
  2. -
  3. -
  4. -
1000 Förderprogramm
  1. Collaborative Research Center 974 “Communication and Systems Relevance during Liver Injury and Regeneration”
  2. International NRW Research School BioStruct
  3. -
  4. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Research Foundation (Deutsche Forschungsgemeinschaft or DFG) |
    1000 Förderprogramm Collaborative Research Center 974 “Communication and Systems Relevance during Liver Injury and Regeneration”
    1000 Fördernummer SFB 974
  2. 1000 joinedFunding-child
    1000 Förderer Ministry of Innovation, Science and Research of the State North Rhine-Westphalia |
    1000 Förderprogramm International NRW Research School BioStruct
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Heinrich-Heine-University of Düsseldorf |
    1000 Förderprogramm -
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer Entrepreneur Foundation at the Heinrich-Heine-University of Düsseldorf |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6404830.rdf
1000 Erstellt am 2017-10-05T13:13:54.229+0200
1000 Erstellt von 122
1000 beschreibt frl:6404830
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Thu Jan 30 20:49:21 CET 2020
1000 Objekt bearb. Mon May 28 10:58:26 CEST 2018
1000 Vgl. frl:6404830
1000 Oai Id
  1. oai:frl.publisso.de:frl:6404830 |
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