WeightNameValue
1000 Titel
  • Cell Volume Kinetics of Adherent Epithelial Cells Measured by Laser Scanning Reflection Microscopy: Determination of Water Permeability Changes of Renal Principal Cells
1000 Autor/in
  1. Maric, Kenan |
  2. Wiesner, Burkhard |
  3. Lorenz, Dorothea |
  4. Klussmann, Enno |
  5. Betz, Thomas |
  6. Rosenthal, Walter |
1000 Erscheinungsjahr 2009
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2009-01-06
1000 Erschienen in
1000 Quellenangabe
  • 80(4): 1783-1790
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1301367/ |
  • https://doi.org/10.1016/S0006-3495(01)76148-6 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The water channel aquaporin-2 (AQP2), a key component of the antidiuretic machinery in the kidney, is rapidly regulated by the antidiuretic hormone vasopressin. The hormone exerts its action by inducing a translocation of AQP2 from intracellular vesicles to the cell membrane. This step requires the elevation of intracellular cyclic AMP. We describe here a new method, laser scanning reflection microscopy (LSRM), suitable for determining cellular osmotic water permeability coefficient changes in primary cultured inner medullary collecting duct (IMCD) cells. The recording of vertical-reflection-mode x-z-scan section areas of unstained, living IMCD cells proved useful and valid for the investigation of osmotic water permeability changes. The time-dependent increases of reflection-mode x-z-scan section areas of swelling cells were fitted to a single-exponential equation. The analysis of the time constants of these processes indicates a twofold increase in osmotic water permeability of IMCD cells after treatment of the cells both with forskolin, a cyclic AMP-elevating agent, and with Clostridium difficile toxin B, an inhibitor of Rho proteins that leads to depolymerization of F-actin-containing stress fibers. This indicates that both agents lead to the functional insertion of AQP2 into the cell membrane. Thus, we have established a new functional assay for the study of the regulation of the water permeability at the cellular level.
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/TWFyaWMsIEtlbmFu|https://frl.publisso.de/adhoc/creator/V2llc25lciwgQnVya2hhcmQ=|https://frl.publisso.de/adhoc/creator/TG9yZW56LCBEb3JvdGhlYQ==|https://frl.publisso.de/adhoc/creator/S2x1c3NtYW5uLCBFbm5v|https://frl.publisso.de/adhoc/creator/QmV0eiwgVGhvbWFz|https://frl.publisso.de/adhoc/creator/Um9zZW50aGFsLCBXYWx0ZXI=
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
  2. - |
1000 Fördernummer
  1. Ro 597/6–3
  2. -
1000 Förderprogramm
  1. -
  2. Fonds der Chemischen Industrie
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer Ro 597/6–3
  2. 1000 joinedFunding-child
    1000 Förderer - |
    1000 Förderprogramm Fonds der Chemischen Industrie
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6405139.rdf
1000 Erstellt am 2017-10-23T11:43:19.535+0200
1000 Erstellt von 25
1000 beschreibt frl:6405139
1000 Bearbeitet von 288
1000 Zuletzt bearbeitet Thu Apr 01 13:15:14 CEST 2021
1000 Objekt bearb. Thu Apr 01 13:15:14 CEST 2021
1000 Vgl. frl:6405139
1000 Oai Id
  1. oai:frl.publisso.de:frl:6405139 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
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