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1000 Titel
  • Circulating microRNAs are deregulated in overweight/obese children: Preliminary results of the I.Family study
1000 Autor/in
  1. Iacomino, Giuseppe |
  2. Russo, Paola |
  3. Stillitano, Ilaria |
  4. Lauria, Fabio |
  5. Marena, Pasquale |
  6. De Luca, Pasquale |
  7. Siani, Alfonso |
  8. Ahrens, Wolfgang |
1000 Erscheinungsjahr 2016
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-03-21
1000 Erschienen in
1000 Quellenangabe
  • 11:7
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • http://dx.doi.org/10.1186/s12263-016-0525-3 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968450/ |
1000 Ergänzendes Material
  • https://genesandnutrition.biomedcentral.com/articles/10.1186/s12263-016-0525-3#Declarations |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: MicroRNAs (miRNAs) are small non-coding RNAs involved in the modulation of gene expression and in the control of numerous cell functions. Alterations of miRNA patterns frequently occur in cancer and metabolic disorders, including obesity. Recent studies showed remarkable stability of miRNAs in both plasma and serum making them suitable as potential circulating biomarkers for a variety of diseases and conditions. The aim of this study was to assess the profile of circulating miRNAs expressed in plasma samples of overweight or obese (OW/Ob) and normal weight (NW) prepubertal children from a European cohort (www.ifamilystudy.eu). The project, aimed to assess the determinants of eating behavior in children and adolescents of eight European countries, is built on the IDEFICS cohort (www.ideficsstudy.eu), established in 2006. Among the participants of the I.Family Italian Cohort, ten OW/Ob (age 10.7 ± 1.5 years, BMI 31.6 ± 4.3 kg/m(2)) and ten NW (age 10.5 ± 2.7 years, BMI 16.4 ± 1.7 kg/m(2)) children were selected for the study. Gene arrays were employed to differentially screen the expression of 372 miRNAs in pooled plasma samples. Deregulated miRNAs (p < 0.05) were further validated in the individual samples using a real-time PCR (RT-qPCR) approach. RESULTS: Using a significance threshold of p < 0.05 and a fold-change threshold of ± 4.0, we preliminarily identified in the pooled samples eight miRNAs that differed between the OW/Ob and NW groups. The validation by RT-qPCR in the individual plasma samples showed a twofold upregulation of miR-31-5p, a threefold upregulation of miR-2355-5p, and a 0.5-fold downregulation of miR-206 in OW/Ob as compared with NW. The molecular functions of these differentially expressed plasma miRNAs as well as their expected mRNA targets were predicted by bioinformatics tools. CONCLUSIONS: This pilot study shows that three circulating miRNAs are differentially regulated in OW/Ob as compared with NW children. Although causal pathways cannot be firmly inferred by these results, that deserve confirmation in larger samples, it is conceivable that circulating miRNAs may be novel biomarkers of obesity and related metabolic disturbances.
1000 Sacherschließung
lokal metabolic disorders
lokal circulating miRNAs
lokal childhood obesity
lokal biomarker
1000 Fachgruppe
  1. Medizin |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/SWFjb21pbm8sIEdpdXNlcHBl|https://frl.publisso.de/adhoc/creator/UnVzc28sIFBhb2xh|https://frl.publisso.de/adhoc/creator/U3RpbGxpdGFubywgSWxhcmlh|https://frl.publisso.de/adhoc/creator/TGF1cmlhLCBGYWJpbw==|https://frl.publisso.de/adhoc/creator/TWFyZW5hLCBQYXNxdWFsZQ==|https://frl.publisso.de/adhoc/creator/RGUgTHVjYSwgUGFzcXVhbGU=|https://frl.publisso.de/adhoc/creator/U2lhbmksIEFsZm9uc28=|http://orcid.org/0000-0003-3777-570X
1000 Förderer
  1. European Community |
1000 Fördernummer
  1. 016181 (FOOD); 266044
1000 Förderprogramm
  1. Sixth RTD Framework Programme; Seventh RTD Framework Programme
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer European Community |
    1000 Förderprogramm Sixth RTD Framework Programme; Seventh RTD Framework Programme
    1000 Fördernummer 016181 (FOOD); 266044
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6405208.rdf
1000 Erstellt am 2017-10-26T13:02:50.239+0200
1000 Erstellt von 122
1000 beschreibt frl:6405208
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Mon May 07 11:46:52 CEST 2018
1000 Objekt bearb. Mon May 07 11:46:52 CEST 2018
1000 Vgl. frl:6405208
1000 Oai Id
  1. oai:frl.publisso.de:frl:6405208 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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