kloth1.pdf 570,33KB
1000 Titel
  • HMGA2 expression distinguishes between different types of postpubertal testicular germ cell tumour
1000 Autor/in
  1. Kloth, Lars |
  2. Gottlieb, Andrea |
  3. Helmke, Burkhard |
  4. Wosniok, Werner |
  5. Löning, Thomas |
  6. Burchardt, Käte |
  7. Belge, Gazanfer |
  8. Günther, Kathrin |
  9. Bullerdiek, Jörn |
1000 Erscheinungsjahr 2015
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2015-09-12
1000 Erschienen in
1000 Quellenangabe
  • 1(4): 239-251
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2015
1000 Lizenz
1000 Verlagsversion
  • |
  • |
1000 Ergänzendes Material
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The group of postpubertal testicular germ cell tumours encompasses lesions with highly diverse differentiation – seminomas, embryonal carcinomas, yolk sac tumours, teratomas and choriocarcinomas. Heterogeneous differentiation is often present within individual tumours and the correct identification of the components is of clinical relevance. HMGA2 re‐expression has been reported in many tumours, including testicular germ cell tumours. This is the first study investigating HMGA2 expression in a representative group of testicular germ cell tumours with the highly sensitive method of quantitative real‐time PCR as well as with immunohistochemistry. The expression of HMGA2 and HPRT was measured using quantitative real‐time PCR in 59 postpubertal testicular germ cell tumours. Thirty specimens contained only one type of tumour and 29 were mixed neoplasms. With the exception of choriocarcinomas, at least two pure specimens from each subgroup of testicular germ cell tumour were included. In order to validate the quantitative real‐time PCR data and gather information about the localisation of the protein, additional immunohistochemical analysis with an antibody specific for HMGA2 was performed in 23 cases. Expression of HMGA2 in testicular germ cell tumours depended on the histological differentiation. Seminomas and embryonal carcinomas showed no or very little expression, whereas yolk sac tumours strongly expressed HMGA2 at the transcriptome as well as the protein level. In teratomas, the expression varied and in choriocarcinomas the expression was moderate. In part, these results contradict data from previous studies but HMGA2 seems to represent a novel marker to assist pathological subtyping of testicular germ cell tumours. The results indicate a critical role in yolk sac tumours and some forms of teratoma.
1000 Sacherschließung
lokal qRT-PCR
lokal HMGA2
lokal testicular germ cell tumour
lokal embryonal carcinoma
lokal immunohistochemistry
lokal seminoma
lokal biomarker
lokal teratoma
lokal yolk sac tumour
lokal choriocarcinoma
1000 Fachgruppe
  1. Medizin |
  2. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
1000 Label
1000 Dateien
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1000 @id frl:6405216.rdf
1000 Erstellt am 2017-10-26T14:20:50.011+0200
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