1000 Titel
  • Involvement of the L-Type Amino Acid Transporter Lat2 in the Transport of 3,3′-Diiodothyronine across the Plasma Membrane
1000 Autor/in
  1. Kinne, Anita |
  2. Wittner, Melanie |
  3. Wirth, Eva K. |
  4. Hinz, Katrin M. |
  5. Schülein, Ralf |
  6. Köhrle, Josef |
  7. Krause, Gerd |
1000 Erscheinungsjahr 2015
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2015-05-28
1000 Erschienen in
1000 Quellenangabe
  • 4(Suppl 1): 42-50
1000 FRL-Sammlung
1000 Verlagsversion
  • http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640293/ |
  • http://dx.doi.org/10.1159/000381542 |
1000 Ergänzendes Material
  • https://dx.doi.org/10.6084/m9.figshare.5127880 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Thyroid hormones are transported across cell membranes by transmembrane transporter proteins, for example by members of the monocarboxylate transporter (MCT) and the L-type amino acid transporter (LAT) families. LATs consist of a light chain (e.g. LAT2) and a heavy chain (CD98), which is essential for their cell surface expression and functionality. The specificity of Lat2 for thyroid hormones and their metabolites and its role in their transport was not fully clear. This fact motivated us to establish a cell system to elucidate the uptake of thyroid hormones and their metabolites by mouse Lat2. The coinjection of cRNA coding for Lat2 and CD98 into Xenopus laevis oocytes resulted in a markedly increased level of 3,3′-diiodo-L-thyronine (3,3′-T2) and to some extent also enhanced T3 transport. To gain insight into properties of thyroid hormones and their metabolites transported by Lat2, we inhibited 3,3′-T2 uptake by various iodothyronine derivatives. T1 and T2 derivatives as well as 2-aminobicyclo-[2, 2,1]-heptane-2-carboxylic acid strongly competed with 3,3′-T2 uptake. In addition, we performed T2 uptake measurements with the thyroid hormone-specific transporter MCT8. For both Lat2 and MCT8, Km values in a low micromolar range were calculated. We demonstrated that oocytes are a suitable system for thyroid hormone transport studies mediated by Lat2. Our data indicates that Lat2 compared to other thyroid hormone transporters prefers 3,3′-T2 as the substrate. Thus, Lat2 might contribute to the availability of thyroid hormone by importing and/or exporting 3,3′-T2, which is generated either by T3 inactivation or by rapid deiodinase 1-mediated rT3 degradation.
1000 Sacherschließung
lokal T3
lokal Thyroid hormones
lokal Oocytes
lokal L-type amino acid transporter 2
lokal 3,3′-Diiodothyronine
lokal Molecular uptake
lokal Transporters
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/S2lubmUsIEFuaXRh|https://frl.publisso.de/adhoc/creator/V2l0dG5lciwgTWVsYW5pZQ==|https://frl.publisso.de/adhoc/creator/V2lydGgsIEV2YSBLLg==|https://frl.publisso.de/adhoc/creator/SGlueiwgS2F0cmluIE0u|https://frl.publisso.de/adhoc/creator/U2Now7xsZWluLCBSYWxm|https://frl.publisso.de/adhoc/creator/S8O2aHJsZSwgSm9zZWY=|https://frl.publisso.de/adhoc/creator/S3JhdXNlLCBHZXJk
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft DFG |
1000 Fördernummer
  1. Ki1751/1-1; Kr1273/5-1; WI3768/2-1; KO 922/17-1
1000 Förderprogramm
  1. Project SPP Thyroid Trans Act 1629
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft DFG |
    1000 Förderprogramm Project SPP Thyroid Trans Act 1629
    1000 Fördernummer Ki1751/1-1; Kr1273/5-1; WI3768/2-1; KO 922/17-1
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6405486.rdf
1000 Erstellt am 2017-11-24T11:15:15.808+0100
1000 Erstellt von 122
1000 beschreibt frl:6405486
1000 Bearbeitet von 288
1000 Zuletzt bearbeitet Thu Aug 18 07:48:56 CEST 2022
1000 Objekt bearb. Wed Jan 27 11:40:25 CET 2021
1000 Vgl. frl:6405486
1000 Oai Id
  1. oai:frl.publisso.de:frl:6405486 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
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