WeightNameValue
1000 Titel
  • Development of an antibody-based, modular biosensor for 129Xe NMR molecular imaging of cells at nanomolar concentrations
1000 Autor/in
  1. Rose, Honor M. |
  2. Witte, Christopher |
  3. Klippel, Stefan |
  4. Freund, Christian |
  5. Rossella, Federica |
  6. Schröder, Leif |
1000 Erscheinungsjahr 2014
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2014-07-28
1000 Erschienen in
1000 Quellenangabe
  • 111(32): 11697–11702
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4136558/ |
  • http://doi.org/10.1073/pnas.1406797111 |
1000 Ergänzendes Material
  • http://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1406797111/-/DCSupplemental |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Magnetic resonance imaging (MRI) is seriously limited when aiming for visualization of targeted contrast agents. Images are reconstructed from the weak diamagnetic properties of the sample and require an abundant molecule like water as the reporter. Micromolar to millimolar concentrations of conventional contrast agents are needed to generate image contrast, thus excluding many molecular markers as potential targets. To address this limitation, we developed and characterized a functional xenon NMR biosensor that can identify a specific cell surface marker by targeted 129Xe MRI. Cells expressing the cell surface protein CD14 can be spatially distinguished from control cells with incorporation of as little as 20 nM of the xenon MRI readout unit, cryptophane-A. Cryptophane-A serves as a chemical host for hyperpolarized nuclei and facilitates the sensitivity enhancement achieved by xenon MRI. Although this paper describes the application of a CD14-specific biosensor, the construct has been designed in a versatile, modular fashion. This allows for quick and easy adaptation of the biosensor to any cell surface target for which there is a specific antibody. In addition, the modular design facilitates the creation of a multifunctional probe that incorporates readout modules for different detection methods, such as fluorescence, to complement the primary MRI readout. This modular antibody-based approach not only offers a practical technique with which to screen targets, but one which can be readily applied as the xenon MRI field moves closer to molecular imaging applications in vivo.
1000 Sacherschließung
lokal targeted imaging
lokal spin hyperpolarization
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/Um9zZSwgSG9ub3IgTS4=|https://frl.publisso.de/adhoc/creator/V2l0dGUsIENocmlzdG9waGVy|https://frl.publisso.de/adhoc/creator/S2xpcHBlbCwgU3RlZmFu|https://frl.publisso.de/adhoc/creator/RnJldW5kLCBDaHJpc3RpYW4=|http://orcid.org/0000-0001-7788-2409|http://orcid.org/0000-0003-4901-0325
1000 Förderer
  1. European Research Council (ERC) |
  2. Leibniz Association (Wissenschaftsgemeinschaft Gottfried Wilhelm Leibniz e.V.) |
  3. Federal Ministry of Education and Research (BMBF) |
  4. - |
1000 Fördernummer
  1. FP7/2007-2013; 242710
  2. SAW-2011-FMP-2
  3. 13EZ1010B
  4. -
1000 Förderprogramm
  1. European Community’s Seventh Framework Programme/ERC Grant Agreement
  2. -
  3. -
  4. Human Frontier Science Program
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer European Research Council (ERC) |
    1000 Förderprogramm European Community’s Seventh Framework Programme/ERC Grant Agreement
    1000 Fördernummer FP7/2007-2013; 242710
  2. 1000 joinedFunding-child
    1000 Förderer Leibniz Association (Wissenschaftsgemeinschaft Gottfried Wilhelm Leibniz e.V.) |
    1000 Förderprogramm -
    1000 Fördernummer SAW-2011-FMP-2
  3. 1000 joinedFunding-child
    1000 Förderer Federal Ministry of Education and Research (BMBF) |
    1000 Förderprogramm -
    1000 Fördernummer 13EZ1010B
  4. 1000 joinedFunding-child
    1000 Förderer - |
    1000 Förderprogramm Human Frontier Science Program
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6405543.rdf
1000 Erstellt am 2017-11-28T13:41:07.103+0100
1000 Erstellt von 25
1000 beschreibt frl:6405543
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Tue Jul 31 11:47:33 CEST 2018
1000 Objekt bearb. Tue Jul 31 11:47:33 CEST 2018
1000 Vgl. frl:6405543
1000 Oai Id
  1. oai:frl.publisso.de:frl:6405543 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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