WeightNameValue
1000 Titel
  • In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice
1000 Autor/in
  1. Fischer, Stefanie |
  2. Engelmann, Christian |
  3. Herrmann, Karl-Heinz |
  4. Reichenbach, Jürgen R. |
  5. Witte, Otto W. |
  6. Weih, Falk |
  7. Kretz, Alexandra |
  8. Haenold, Ronny |
1000 Erscheinungsjahr 2014
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2014-07-22
1000 Erschienen in
1000 Quellenangabe
  • 89: 51274
1000 FRL-Sammlung
1000 Verlagsversion
  • http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4318583/ |
  • http://dx.doi.org/10.3791/51274 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The rodent visual system encompasses retinal ganglion cells and their axons that form the optic nerve to enter thalamic and midbrain centers, and postsynaptic projections to the visual cortex. Based on its distinct anatomical structure and convenient accessibility, it has become the favored structure for studies on neuronal survival, axonal regeneration, and synaptic plasticity. Recent advancements in MR imaging have enabled the in vivo visualization of the retino-tectal part of this projection using manganese mediated contrast enhancement (MEMRI). Here, we present a MEMRI protocol for illustration of the visual projection in mice, by which resolutions of (200 µm)3 can be achieved using common 3 Tesla scanners. We demonstrate how intravitreal injection of a single dosage of 15 nmol MnCl2 leads to a saturated enhancement of the intact projection within 24 hr. With exception of the retina, changes in signal intensity are independent of coincided visual stimulation or physiological aging. We further apply this technique to longitudinally monitor axonal degeneration in response to acute optic nerve injury, a paradigm by which Mn2+ transport completely arrests at the lesion site. Conversely, active Mn2+ transport is quantitatively proportionate to the viability, number, and electrical activity of axon fibers. For such an analysis, we exemplify Mn2+ transport kinetics along the visual path in a transgenic mouse model (NF-κB p50KO) displaying spontaneous atrophy of sensory, including visual, projections. In these mice, MEMRI indicates reduced but not delayed Mn2+ transport as compared to wild type mice, thus revealing signs of structural and/or functional impairments by NF-κB mutations. In summary, MEMRI conveniently bridges in vivo assays and post mortem histology for the characterization of nerve fiber integrity and activity. It is highly useful for longitudinal studies on axonal degeneration and regeneration, and investigations of mutant mice for genuine or inducible phenotypes.
1000 Sacherschließung
lokal manganese-enhanced MRI
lokal visual system
lokal NF-κB
lokal Neuroscience
lokal mouse retino-tectal projection
lokal Issue 89
lokal neurodegeneration
lokal optic nerve injury
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/RmlzY2hlciwgU3RlZmFuaWU=|https://frl.publisso.de/adhoc/creator/RW5nZWxtYW5uLCBDaHJpc3RpYW4=|https://frl.publisso.de/adhoc/creator/SGVycm1hbm4sIEthcmwtSGVpbno=|https://frl.publisso.de/adhoc/creator/UmVpY2hlbmJhY2gsIErDvHJnZW4gUi4=|https://frl.publisso.de/adhoc/creator/V2l0dGUsIE90dG8gVy4=|https://frl.publisso.de/adhoc/creator/V2VpaCwgRmFsaw==|https://frl.publisso.de/adhoc/creator/S3JldHosIEFsZXhhbmRyYQ==|https://frl.publisso.de/adhoc/creator/SGFlbm9sZCwgUm9ubnk=
1000 Förderer
  1. Oppenheim Foundation |
  2. Velux Foundation |
1000 Fördernummer
  1. -
  2. -
1000 Förderprogramm
  1. -
  2. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Oppenheim Foundation |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Velux Foundation |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6405545.rdf
1000 Erstellt am 2017-11-28T15:04:56.382+0100
1000 Erstellt von 25
1000 beschreibt frl:6405545
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Mon May 28 09:56:37 CEST 2018
1000 Objekt bearb. Mon May 28 09:56:36 CEST 2018
1000 Vgl. frl:6405545
1000 Oai Id
  1. oai:frl.publisso.de:frl:6405545 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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