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WeightNameValue
1000 Titel
  • Vesicle uncoating regulated by SH3‐SH3 domain‐mediated complex formation between endophilin and intersectin at synapses
1000 Autor/in
  1. Pechstein, Arndt |
  2. Gerth, Fabian |
  3. Milosevic, Ira |
  4. Jäpel, Maria |
  5. Eichhorn-Grünig, Marielle |
  6. Vorontsova, Olga |
  7. Bacetic, Jelena |
  8. Shupliakov, Oleg |
  9. Freund, Christian |
  10. Maritzen, Tanja |
  11. Haucke, Volker |
1000 Erscheinungsjahr 2014
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2014-12-17
1000 Erschienen in
1000 Quellenangabe
  • 16(2): 232-239
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2014
1000 Lizenz
1000 Verlagsversion
  • https://dx.doi.org/10.15252/embr.201439260 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4328750/ |
1000 Ergänzendes Material
  • http://embor.embopress.org/content/16/2/232#sec-12 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Neurotransmission involves the exo‐endocytic cycling of synaptic vesicle (SV) membranes. Endocytic membrane retrieval and clathrin‐mediated SV reformation require curvature‐sensing and membrane‐bending BAR domain proteins such as endophilin A. While their ability to sense and stabilize curved membranes facilitates membrane recruitment of BAR domain proteins, the precise mechanisms by which they are targeted to specific sites of SV recycling has remained unclear. Here, we demonstrate that the multi‐domain scaffold intersectin 1 directly associates with endophilin A to facilitate vesicle uncoating at synapses. Knockout mice deficient in intersectin 1 accumulate clathrin‐coated vesicles at synapses, a phenotype akin to loss of endophilin function. Intersectin 1/endophilin A1 complex formation is mediated by direct binding of the SH3B domain of intersectin to a non‐canonical site on the SH3 domain of endophilin A1. Consistent with this, intersectin‐binding defective mutant endophilin A1 fails to rescue clathrin accumulation at neuronal synapses derived from endophilin A1‐3 triple knockout (TKO) mice. Our data support a model in which intersectin aids endophilin A recruitment to sites of clathrin‐mediated SV recycling, thereby facilitating vesicle uncoating.
1000 Sacherschließung
lokal endophilin
lokal SH3 domains
lokal intersectin
lokal neurotransmission
lokal synaptic vesicle recycling
1000 Fachgruppe
  1. Medizin |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/UGVjaHN0ZWluLCBBcm5kdA==|https://frl.publisso.de/adhoc/creator/R2VydGgsIEZhYmlhbg==|https://frl.publisso.de/adhoc/creator/TWlsb3NldmljLCBJcmE=|https://frl.publisso.de/adhoc/creator/SsOkcGVsLCBNYXJpYQ==|https://frl.publisso.de/adhoc/creator/RWljaGhvcm4tR3LDvG5pZywgTWFyaWVsbGU=|https://frl.publisso.de/adhoc/creator/Vm9yb250c292YSwgT2xnYQ==|https://frl.publisso.de/adhoc/creator/QmFjZXRpYywgSmVsZW5h|https://frl.publisso.de/adhoc/creator/U2h1cGxpYWtvdiwgT2xlZw==|https://frl.publisso.de/adhoc/creator/RnJldW5kLCBDaHJpc3RpYW4=|http://orcid.org/0000-0003-4993-0340|http://orcid.org/0000-0003-3119-6993
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft (DFG) |
  2. Swedish Research Council |
  3. European Union |
  4. EMBO |
1000 Fördernummer
  1. SFB958/A07
  2. 13473
  3. HEALTH‐F2‐2009‐242167
  4. -
1000 Förderprogramm
  1. Emmy Noether Award
  2. -
  3. FP7: SynSys‐project
  4. long‐term fellowship
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft (DFG) |
    1000 Förderprogramm Emmy Noether Award
    1000 Fördernummer SFB958/A07
  2. 1000 joinedFunding-child
    1000 Förderer Swedish Research Council |
    1000 Förderprogramm -
    1000 Fördernummer 13473
  3. 1000 joinedFunding-child
    1000 Förderer European Union |
    1000 Förderprogramm FP7: SynSys‐project
    1000 Fördernummer HEALTH‐F2‐2009‐242167
  4. 1000 joinedFunding-child
    1000 Förderer EMBO |
    1000 Förderprogramm long‐term fellowship
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6405572.rdf
1000 Erstellt am 2017-11-29T19:05:38.779+0100
1000 Erstellt von 218
1000 beschreibt frl:6405572
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Thu Jan 30 18:26:17 CET 2020
1000 Objekt bearb. Wed Aug 01 12:25:33 CEST 2018
1000 Vgl. frl:6405572
1000 Oai Id
  1. oai:frl.publisso.de:frl:6405572 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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