WeightNameValue
1000 Titel
  • A Sequence-Ready Physical Map of Barley Anchored Genetically by Two Million Single-Nucleotide Polymorphisms
1000 Autor/in
  1. Ariyadasa, Ruvini |
  2. Mascher, Martin |
  3. Nussbaumer, Thomas |
  4. Schulte, Daniela |
  5. Frenkel, Zeev |
  6. Poursarebani, Naser |
  7. Zhou, Ruonan |
  8. Steuernagel, Burkhard |
  9. Gundlach, Heidrun |
  10. Taudien, Stefan |
  11. Felder, Marius |
  12. Platzer, Matthias |
  13. Himmelbach, Axel |
  14. Schmutzer, Thomas |
  15. Hedley, Pete E. |
  16. Muehlbauer, Gary J. |
  17. Scholz, Uwe |
  18. Korol, Abraham |
  19. Mayer, Klaus F.X. |
  20. Waugh, Robbie |
  21. Langridge, Peter |
  22. Graner, Andreas |
  23. Stein, Nils |
1000 Erscheinungsjahr 2014
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2014-01
1000 Erschienen in
1000 Quellenangabe
  • 164: 412–423
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875818/ |
  • https://doi.org/10.1104/pp.113.228213 |
1000 Ergänzendes Material
  • http://www.plantphysiol.org/content/164/1/412/tab-figures-data |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Barley (Hordeum vulgare) is an important cereal crop and a model species for Triticeae genomics. To lay the foundation for hierarchical map-based sequencing, a genome-wide physical map of its large and complex 5.1 billion-bp genome was constructed by high-information content fingerprinting of almost 600,000 bacterial artificial chromosomes representing 14-fold haploid genome coverage. The resultant physical map comprises 9,265 contigs with a cumulative size of 4.9 Gb representing 96% of the physical length of the barley genome. The reliability of the map was verified through extensive genetic marker information and the analysis of topological networks of clone overlaps. A minimum tiling path of 66,772 minimally overlapping clones was defined that will serve as a template for hierarchical clone-by-clone map-based shotgun sequencing. We integrated whole-genome shotgun sequence data from the individuals of two mapping populations with published bacterial artificial chromosome survey sequence information to genetically anchor the physical map. This novel approach in combination with the comprehensive whole-genome shotgun sequence data sets allowed us to independently validate and improve a previously reported physical and genetic framework. The resources developed in this study will underpin fine-mapping and cloning of agronomically important genes and the assembly of a draft genome sequence.
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/QXJpeWFkYXNhLCBSdXZpbmk=|http://orcid.org/0000-0001-6373-6013|https://frl.publisso.de/adhoc/creator/TnVzc2JhdW1lciwgVGhvbWFz|https://frl.publisso.de/adhoc/creator/U2NodWx0ZSwgRGFuaWVsYQ==|https://frl.publisso.de/adhoc/creator/RnJlbmtlbCwgWmVldg==|https://frl.publisso.de/adhoc/creator/UG91cnNhcmViYW5pLCBOYXNlcg==|http://orcid.org/0000-0002-1909-3564|http://orcid.org/0000-0002-8284-7728|https://frl.publisso.de/adhoc/creator/R3VuZGxhY2gsIEhlaWRydW4=|https://frl.publisso.de/adhoc/creator/VGF1ZGllbiwgU3RlZmFu|https://frl.publisso.de/adhoc/creator/RmVsZGVyLCBNYXJpdXM=|http://orcid.org/0000-0003-0596-8582|http://orcid.org/0000-0001-7338-0946|http://orcid.org/0000-0003-1073-6719|https://frl.publisso.de/adhoc/creator/SGVkbGV5LCBQZXRlIEUu|https://frl.publisso.de/adhoc/creator/TXVlaGxiYXVlciwgR2FyeSBKLg==|http://orcid.org/0000-0001-6113-3518|https://frl.publisso.de/adhoc/creator/S29yb2wsIEFicmFoYW0=|https://frl.publisso.de/adhoc/creator/TWF5ZXIsIEtsYXVzIEYuWC4=|https://frl.publisso.de/adhoc/creator/V2F1Z2gsIFJvYmJpZQ==|https://frl.publisso.de/adhoc/creator/TGFuZ3JpZGdlLCBQZXRlcg==|http://orcid.org/0000-0003-3246-6393|http://orcid.org/0000-0003-3011-8731
1000 Objektart article
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1000 @id frl:6405590.rdf
1000 Erstellt am 2017-11-30T11:43:00.105+0100
1000 Erstellt von 25
1000 beschreibt frl:6405590
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Mon Apr 30 12:54:21 CEST 2018
1000 Objekt bearb. Mon Apr 30 12:54:20 CEST 2018
1000 Vgl. frl:6405590
1000 Oai Id
  1. oai:frl.publisso.de:frl:6405590 |
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