12979_2014_Article_17.pdf 1,35MB
1000 Titel
  • SENIEUR status of the originating cell donor negates certain ‘anti-immunosenescence’ effects of ebselen and N-acetyl cysteine in human T cell clone cultures
1000 Autor/in
  1. Marthandan, Shiva |
  2. Freeburn, Robin |
  3. Steinbrecht, Susanne |
  4. Pawelec, Graham |
  5. Barnett, Yvonne |
1000 Erscheinungsjahr 2014
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2014-11-30
1000 Erschienen in
1000 Quellenangabe
  • 11: 17
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2014
1000 Lizenz
1000 Verlagsversion
  • |
  • |
1000 Ergänzendes Material
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: Damage to T cells of the immune system by reactive oxygen species may result in altered cell function or cell death and thereby potentially impact upon the efficacy of a subsequent immune response. Here, we assess the impact of the antioxidants Ebselen and N-acetyl cysteine on a range of biological markers in human T cells derived from a SENIEUR status donor. In addition, the impact of these antioxidants on different MAP kinase pathways in T cells from donors of different ages was also examined. METHODS: T cell clones were derived from healthy 26, 45 and SENIEUR status 80 year old people and the impact of titrated concentrations of Ebselen or N-acetyl cysteine on their proliferation and in vitro lifespan, GSH:GSSG ratio as well as levels of oxidative DNA damage and on MAP kinase signaling pathways was examined. RESULTS: In this investigation neither Ebselen nor N-acetyl cysteine supplementation had any impact on the biological endpoints examined in the T cells derived from the SENIEUR status 80 year old donor. This is in contrast to the anti-immunosenescent effects of these antioxidants on T cells from donors of 26 or 45 years of age. The analysis of MAP kinases showed that pro-apoptotic pathways become activated in T cells with increasing in vitro age and that Ebselen or N-acetyl cysteine could decrease activation (phosphorylation) in T cells from 26 or 45 year old donors, but not from the SENIEUR status 80 year old donor. CONCLUSIONS: The results of this investigation demonstrate that the biological phenotype of SENIEUR status derived human T cells negates the anti-immunosenescence effects of Ebselen and also N-acetyl cysteine. The results highlight the importance of pre-antioxidant intervention evaluation to determine risk-benefit.
1000 Sacherschließung
lokal DNA damage
lokal JNK
lokal Total glutathione
lokal Ebselen
lokal NAC
lokal p38
lokal GSH:GSSG ratio
lokal MAP kinases
lokal Immunosenescence
lokal Proliferative capacity
lokal Lifespan
lokal ERK
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
1000 Förderer
  1. German Ministry for Education and Research (BMBF) |
1000 Fördernummer
  1. 0315581
1000 Förderprogramm
  1. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Ministry for Education and Research (BMBF) |
    1000 Förderprogramm -
    1000 Fördernummer 0315581
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6405864.rdf
1000 Erstellt am 2017-12-14T16:06:42.662+0100
1000 Erstellt von 218
1000 beschreibt frl:6405864
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Wed Jun 20 12:13:23 CEST 2018
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1000 Vgl. frl:6405864
1000 Oai Id
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