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1000 Titel
  • SENIEUR status of the originating cell donor negates certain ‘anti-immunosenescence’ effects of ebselen and N-acetyl cysteine in human T cell clone cultures
1000 Autor/in
  1. Marthandan, Shiva |
  2. Freeburn, Robin |
  3. Steinbrecht, Susanne |
  4. Pawelec, Graham |
  5. Barnett, Yvonne |
1000 Erscheinungsjahr 2014
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2014-11-30
1000 Erschienen in
1000 Quellenangabe
  • 11: 17
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2014
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1186/s12979-014-0017-5 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263119/ |
1000 Ergänzendes Material
  • https://immunityageing.biomedcentral.com/articles/10.1186/s12979-014-0017-5#Sec16 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: Damage to T cells of the immune system by reactive oxygen species may result in altered cell function or cell death and thereby potentially impact upon the efficacy of a subsequent immune response. Here, we assess the impact of the antioxidants Ebselen and N-acetyl cysteine on a range of biological markers in human T cells derived from a SENIEUR status donor. In addition, the impact of these antioxidants on different MAP kinase pathways in T cells from donors of different ages was also examined. METHODS: T cell clones were derived from healthy 26, 45 and SENIEUR status 80 year old people and the impact of titrated concentrations of Ebselen or N-acetyl cysteine on their proliferation and in vitro lifespan, GSH:GSSG ratio as well as levels of oxidative DNA damage and on MAP kinase signaling pathways was examined. RESULTS: In this investigation neither Ebselen nor N-acetyl cysteine supplementation had any impact on the biological endpoints examined in the T cells derived from the SENIEUR status 80 year old donor. This is in contrast to the anti-immunosenescent effects of these antioxidants on T cells from donors of 26 or 45 years of age. The analysis of MAP kinases showed that pro-apoptotic pathways become activated in T cells with increasing in vitro age and that Ebselen or N-acetyl cysteine could decrease activation (phosphorylation) in T cells from 26 or 45 year old donors, but not from the SENIEUR status 80 year old donor. CONCLUSIONS: The results of this investigation demonstrate that the biological phenotype of SENIEUR status derived human T cells negates the anti-immunosenescence effects of Ebselen and also N-acetyl cysteine. The results highlight the importance of pre-antioxidant intervention evaluation to determine risk-benefit.
1000 Sacherschließung
lokal ERK
lokal MAP kinases
lokal p38
lokal Total glutathione
lokal Lifespan
lokal SENIEUR
lokal Proliferative capacity
lokal Ebselen
lokal DNA damage
lokal Immunosenescence
lokal JNK
lokal NAC
lokal GSH:GSSG ratio
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1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/TWFydGhhbmRhbiwgU2hpdmE=|https://frl.publisso.de/adhoc/creator/RnJlZWJ1cm4sIFJvYmlu|https://frl.publisso.de/adhoc/creator/U3RlaW5icmVjaHQsIFN1c2FubmU=|https://frl.publisso.de/adhoc/creator/UGF3ZWxlYywgR3JhaGFt|https://frl.publisso.de/adhoc/creator/QmFybmV0dCwgWXZvbm5l
1000 Label
1000 Förderer
  1. German Ministry for Education and Research (BMBF) |
1000 Fördernummer
  1. 0315581
1000 Förderprogramm
  1. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Ministry for Education and Research (BMBF) |
    1000 Förderprogramm -
    1000 Fördernummer 0315581
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6405864.rdf
1000 Erstellt am 2017-12-14T16:06:42.662+0100
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1000 Zuletzt bearbeitet Fri Dec 04 12:01:18 CET 2020
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