Download
srep08889.pdf 3,40MB
WeightNameValue
1000 Titel
  • Irisin – a myth rather than an exercise-inducible myokine
1000 Autor/in
  1. Norheim, Frode |
  2. Thiede, Bernd |
  3. Holen, Torgeir |
  4. Ohashi, Tomoo |
  5. Schering, Lisa |
  6. Lee, Sindre |
  7. Brenmoehl, Julia |
  8. Thomas, Selina |
  9. Drevon, Christian A. |
  10. Erickson, Harold P. |
  11. Albrecht, Elke |
  12. Maak, Steffen |
1000 Erscheinungsjahr 2015
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2015-03-09
1000 Erschienen in
1000 Quellenangabe
  • 5: 8889
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2015
1000 Lizenz
1000 Verlagsversion
  • http://dx.doi.org/10.1038/srep08889 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4352853/ |
1000 Ergänzendes Material
  • https://www.nature.com/articles/srep08889#supplementary-information |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The myokine irisin is supposed to be cleaved from a transmembrane precursor, FNDC5 (fibronectin type III domain containing 5), and to mediate beneficial effects of exercise on human metabolism. However, evidence for irisin circulating in blood is largely based on commercial ELISA kits which are based on polyclonal antibodies (pAbs) not previously tested for cross-reacting serum proteins. We have analyzed four commercial pAbs by Western blotting, which revealed prominent cross-reactivity with non-specific proteins in human and animal sera. Using recombinant glycosylated and non-glycosylated irisin as positive controls, we found no immune-reactive bands of the expected size in any biological samples. A FNDC5 signature was identified at ~20 kDa by mass spectrometry in human serum but was not detected by the commercial pAbs tested. Our results call into question all previous data obtained with commercial ELISA kits for irisin, and provide evidence against a physiological role for irisin in humans and other species.
1000 Sacherschließung
lokal Transcription
lokal Fat Metabolism
1000 Fachgruppe
  1. Medizin |
  2. Agrarwissenschaften |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/Tm9yaGVpbSwgRnJvZGU=|https://frl.publisso.de/adhoc/creator/VGhpZWRlLCBCZXJuZA==|https://frl.publisso.de/adhoc/creator/SG9sZW4sIFRvcmdlaXI=|https://frl.publisso.de/adhoc/creator/T2hhc2hpLCBUb21vbw==|https://frl.publisso.de/adhoc/creator/U2NoZXJpbmcsIExpc2E=|https://frl.publisso.de/adhoc/creator/TGVlLCBTaW5kcmU=|https://frl.publisso.de/adhoc/creator/QnJlbm1vZWhsLCBKdWxpYQ==|https://frl.publisso.de/adhoc/creator/VGhvbWFzLCBTZWxpbmE=|https://frl.publisso.de/adhoc/creator/RHJldm9uLCBDaHJpc3RpYW4gQS4=|https://frl.publisso.de/adhoc/creator/RXJpY2tzb24sIEhhcm9sZCBQLg==|http://orcid.org/0000-0001-5974-1514|http://orcid.org/0000-0003-2681-1496
1000 Label
1000 Förderer
  1. Leibniz Association |
  2. National Institutes of Health (NIH) |
  3. Institute of Basic Medical Sciences |
  4. University of Oslo (UiO) |
  5. Helse Sør-Øst |
  6. Johan Throne-Holst Foundation for Nutrition Research |
  7. Freia Medical Research Foundation |
  8. European Union |
1000 Fördernummer
  1. SAW-2013-FBN-3
  2. R01-CA47056
  3. -
  4. -
  5. -
  6. -
  7. -
  8. 289511
1000 Förderprogramm
  1. -
  2. -
  3. -
  4. -
  5. -
  6. -
  7. -
  8. FP7 project: NutriTech
1000 Dateien
  1. Irisin – a myth rather than an exercise-inducible myokine
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Leibniz Association |
    1000 Förderprogramm -
    1000 Fördernummer SAW-2013-FBN-3
  2. 1000 joinedFunding-child
    1000 Förderer National Institutes of Health (NIH) |
    1000 Förderprogramm -
    1000 Fördernummer R01-CA47056
  3. 1000 joinedFunding-child
    1000 Förderer Institute of Basic Medical Sciences |
    1000 Förderprogramm -
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer University of Oslo (UiO) |
    1000 Förderprogramm -
    1000 Fördernummer -
  5. 1000 joinedFunding-child
    1000 Förderer Helse Sør-Øst |
    1000 Förderprogramm -
    1000 Fördernummer -
  6. 1000 joinedFunding-child
    1000 Förderer Johan Throne-Holst Foundation for Nutrition Research |
    1000 Förderprogramm -
    1000 Fördernummer -
  7. 1000 joinedFunding-child
    1000 Förderer Freia Medical Research Foundation |
    1000 Förderprogramm -
    1000 Fördernummer -
  8. 1000 joinedFunding-child
    1000 Förderer European Union |
    1000 Förderprogramm FP7 project: NutriTech
    1000 Fördernummer 289511
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6406012.rdf
1000 Erstellt am 2017-12-21T18:46:29.226+0100
1000 Erstellt von 218
1000 beschreibt frl:6406012
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Fri Jan 31 02:42:42 CET 2020
1000 Objekt bearb. Mon May 28 10:24:12 CEST 2018
1000 Vgl. frl:6406012
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406012 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

View source