WeightNameValue
1000 Titel
  • A Novel Subtype of AP-1-binding Motif within the Palmitoylated trans-Golgi Network/Endosomal Accessory Protein Gadkin/γ-BAR
1000 Autor/in
  1. Schmidt, Michael R. |
  2. Kukhtina, Viktoria |
  3. Higman, Victoria A. |
  4. Strauss, Holger |
  5. Volkmer, Rudolf |
  6. Oschkinat, Hartmut |
  7. Dotti, Carlos G. |
  8. Maritzen, Tanja |
  9. Haucke, Volker |
1000 Erscheinungsjahr 2009
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2009-12-03
1000 Erschienen in
1000 Quellenangabe
  • 285: 4074-4086
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2823548/ |
  • http://doi.org/10.1074/jbc.M109.049197 |
1000 Ergänzendes Material
  • http://www.jbc.org/content/285/6/4074/suppl/DC1 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Membrane traffic between the trans-Golgi network (TGN) and endosomes is mediated in part by the assembly of clathrin-AP-1 adaptor complex-coated vesicles. This process involves multiple accessory proteins that directly bind to the ear domain of AP-1γ via degenerate peptide motifs that conform to the consensus sequence ØG(P/D/E)(Ø/L/M) (with Ø being a large hydrophobic amino acid). Recently, γ-BAR (hereafter referred to as Gadkin for reasons explained below) has been identified as a novel AP-1 recruitment factor involved in AP-1-dependent endosomal trafficking of lysosomal enzymes. How precisely Gadkin interacts with membranes and with AP-1γ has remained unclear. Here we show that Gadkin is an S-palmitoylated peripheral membrane protein that lacks stable tertiary structure. S-Palmitoylation is required for the recruitment of Gadkin to TGN/endosomal membranes but not for binding to AP-1. Furthermore, we identify a novel subtype of AP-1-binding motif within Gadkin that specifically associates with the γ1-adaptin ear domain. Mutational inactivation of this novel type of motif, either alone or in combination with three more conventional AP-1γ binding peptides, causes Gadkin to mislocalize to the plasma membrane and interferes with its ability to render AP-1 brefeldin A-resistant, indicating its physiological importance. Our studies thus unravel the molecular basis for Gadkin-mediated AP-1 recruitment to TGN/endosomal membranes and identify a novel subtype of the AP-1-binding motif.
1000 Sacherschließung
lokal Cell/Exocytosis
lokal Protein/Palmitoylation
lokal Cell/Endocytosis
lokal Subcellular Organelles/Endosomes
lokal Membrane/Trafficking
lokal Methods/NMR
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/U2NobWlkdCwgTWljaGFlbCBSLg==|https://frl.publisso.de/adhoc/creator/S3VraHRpbmEsIFZpa3Rvcmlh|https://frl.publisso.de/adhoc/creator/SGlnbWFuLCBWaWN0b3JpYSBBLg==|https://frl.publisso.de/adhoc/creator/U3RyYXVzcywgSG9sZ2Vy|https://frl.publisso.de/adhoc/creator/Vm9sa21lciwgUnVkb2xm|https://frl.publisso.de/adhoc/creator/T3NjaGtpbmF0LCBIYXJ0bXV0|https://frl.publisso.de/adhoc/creator/RG90dGksIENhcmxvcyBHLg==|http://orcid.org/0000-0003-4993-0340|http://orcid.org/0000-0003-3119-6993
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft (DFG) |
1000 Fördernummer
  1. HA2686/1-3; HA2686/1-4; SFB 449/TP A11; SFB 449/TP Z1; Exc 257-Neurocure
1000 Förderprogramm
  1. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft (DFG) |
    1000 Förderprogramm -
    1000 Fördernummer HA2686/1-3; HA2686/1-4; SFB 449/TP A11; SFB 449/TP Z1; Exc 257-Neurocure
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6406070.rdf
1000 Erstellt am 2018-01-02T15:57:27.468+0100
1000 Erstellt von 25
1000 beschreibt frl:6406070
1000 Bearbeitet von 288
1000 Zuletzt bearbeitet Thu Aug 18 07:45:44 CEST 2022
1000 Objekt bearb. Wed Mar 24 13:51:12 CET 2021
1000 Vgl. frl:6406070
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406070 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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