WeightNameValue
1000 Titel
  • Proline-rich sequence recognition: II. Proteomics analysis of Tsg101 ubiquitin-E2-like variant (UEV) interactions
1000 Autor/in
  1. Sticht, Jana |
  2. Piotukh, Kirill |
  3. Kosslick, Daniela |
  4. Jahnke, Nadin |
  5. Keller, Sandro |
  6. Schuemann, Michael |
  7. Krause, Eberhard |
  8. Freund, Christian |
  9. Schlundt, Andreas |
1000 Erscheinungsjahr 2009
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2009-06-20
1000 Erschienen in
1000 Quellenangabe
  • 8: 2474-2486
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2773715/ |
  • http://doi.org/10.1074/mcp.M800337-MCP200 |
1000 Ergänzendes Material
  • http://www.mcponline.org/content/8/11/2474/suppl/DC1 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The tumor maintenance protein Tsg101 has recently gained much attention because of its involvement in endosomal sorting, virus release, cytokinesis, and cancerogenesis. The ubiquitin-E2-like variant (UEV) domain of the protein interacts with proline-rich sequences of target proteins that contain P(S/T)AP amino acid motifs and weakly binds to the ubiquitin moiety of proteins committed to sorting or degradation. Here we performed peptide spot analysis and phage display to refine the peptide binding specificity of the Tsg101 UEV domain. A mass spectrometric proteomics approach that combines domain-based pulldown experiments, binding site inactivation, and stable isotope labeling by amino acids in cell culture (SILAC) was then used to delineate the relative importance of the peptide and ubiquitin binding sites. Clearly “PTAP” interactions dominate target recognition, and we identified several novel binders as for example the poly(A)-binding protein 1 (PABP1), Sec24b, NFκB2, and eIF4b. For PABP1 and eIF4b the interactions were confirmed in the context of the corresponding full-length proteins in cellular lysates. Therefore, our results strongly suggest additional roles of Tsg101 in cellular regulation of mRNA translation. Regulation of Tsg101 itself by the ubiquitin ligase TAL (Tsg101-associated ligase) is most likely conferred by a single PSAP binding motif that enables the interaction with Tsg101 UEV. Together with the results from the accompanying article (Kofler, M., Schuemann, M., Merz, C., Kosslick, D., Schlundt, A., Tannert, A., Schaefer, M., Lührmann, R., Krause, E., and Freund, C. (2009) Proline-rich sequence recognition: I. Marking GYF and WW domain assembly sites in early spliceosomal complexes. Mol. Cell. Proteomics 8, 2461–2473) on GYF and WW domain pathways our work defines major proline-rich sequence-mediated interaction networks that contribute to the modular assembly of physiologically relevant protein complexes.
1000 Fachgruppe
  1. Biologie |
  2. Medizin |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/U3RpY2h0LCBKYW5h|https://frl.publisso.de/adhoc/creator/UGlvdHVraCwgS2lyaWxs|https://frl.publisso.de/adhoc/creator/S29zc2xpY2ssIERhbmllbGE=|https://frl.publisso.de/adhoc/creator/SmFobmtlLCBOYWRpbg==|https://frl.publisso.de/adhoc/creator/S2VsbGVyLCBTYW5kcm8=|https://frl.publisso.de/adhoc/creator/U2NodWVtYW5uLCBNaWNoYWVs|https://frl.publisso.de/adhoc/creator/S3JhdXNlLCBFYmVyaGFyZA==|https://frl.publisso.de/adhoc/creator/RnJldW5kLCBDaHJpc3RpYW4=|http://orcid.org/0000-0003-2254-7560
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
1000 Fördernummer
  1. FG806; SFB740; SFB765
1000 Förderprogramm
  1. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer FG806; SFB740; SFB765
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6406096.rdf
1000 Erstellt am 2018-01-03T08:46:05.088+0100
1000 Erstellt von 25
1000 beschreibt frl:6406096
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Wed Aug 01 12:40:44 CEST 2018
1000 Objekt bearb. Wed Aug 01 12:40:44 CEST 2018
1000 Vgl. frl:6406096
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406096 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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