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1000 Titel
  • Structure of the competence pilus major pilin ComGC in Streptococcus pneumoniae
1000 Autor/in
  1. Muschiol, Sandra |
  2. Erlendsson, Simon |
  3. Aschtgen, Marie-Stephanie |
  4. Oliveira, Vitor |
  5. De Lichtenberg, Casper |
  6. Teilum, Kaare |
  7. Boesen, Thomas |
  8. Akbey, Umit |
  9. Henriques-Normark, Birgitta |
  10. Schmieder, Peter |
1000 Erscheinungsjahr 2017
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-06-28
1000 Erschienen in
1000 Quellenangabe
  • 292(34): 14134-14146
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2017
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1074/jbc.M117.787671 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5572924/ |
1000 Ergänzendes Material
  • http://www.jbc.org/content/292/34/14134/suppl/DC1 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Type IV pili are important virulence factors on the surface of many pathogenic bacteria and have been implicated in a wide range of diverse functions, including attachment, twitching motility, biofilm formation, and horizontal gene transfer. The respiratory pathogen Streptococcus pneumoniae deploys type IV pili to take up DNA during transformation. These “competence pili” are composed of the major pilin protein ComGC and exclusively assembled during bacterial competence, but their biogenesis remains unclear. Here, we report the high resolution NMR structure of N-terminal truncated ComGC revealing a highly flexible and structurally divergent type IV pilin. It consists of only three α-helical segments forming a well-defined electronegative cavity and confined electronegative and hydrophobic patches. The structure is particularly flexible between the first and second α-helix with the first helical part exhibiting slightly slower dynamics than the rest of the pilin, suggesting that the first helix is involved in forming the pilus structure core and that parts of helices two and three are primarily surface-exposed. Taken together, our results provide the first structure of a type IV pilin protein involved in the formation of competence-induced pili in Gram-positive bacteria and corroborate the remarkable structural diversity among type IV pilin proteins.
1000 Sacherschließung
lokal protein structure
lokal pneumococci
lokal nuclear magnetic resonance (NMR)
lokal major pilin
lokal microbiology
lokal transformation
lokal horizontal gene transfer
lokal type IV pili
lokal Streptococcus pneumoniae
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/TXVzY2hpb2wsIFNhbmRyYQ==|https://frl.publisso.de/adhoc/creator/RXJsZW5kc3NvbiwgU2ltb24=|https://frl.publisso.de/adhoc/creator/QXNjaHRnZW4sIE1hcmllLVN0ZXBoYW5pZQ==|https://frl.publisso.de/adhoc/creator/T2xpdmVpcmEsIFZpdG9y|https://frl.publisso.de/adhoc/creator/RGUgTGljaHRlbmJlcmcsIENhc3Blcg==|https://frl.publisso.de/adhoc/creator/VGVpbHVtLCBLYWFyZQ==|https://frl.publisso.de/adhoc/creator/Qm9lc2VuLCBUaG9tYXM=|https://frl.publisso.de/adhoc/creator/QWtiZXksIFVtaXQ=|https://frl.publisso.de/adhoc/creator/SGVucmlxdWVzLU5vcm1hcmssIEJpcmdpdHRh|http://orcid.org/0000-0001-9968-9327
1000 Förderer
  1. Swedish Research Council |
  2. Stockholm County Council |
  3. Karolinska Institutet |
  4. Swedish Foundation for Strategic Research (SSF) |
  5. Knut and Alice Wallenberg Foundation |
  6. Carlsberg Foundation |
  7. Lundbeck Foundation |
1000 Fördernummer
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1000 Förderprogramm
  1. -
  2. regional agreement on medical training and clinical research (ALF)
  3. regional agreement on medical training and clinical research (ALF)
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  5. -
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  7. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Swedish Research Council |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Stockholm County Council |
    1000 Förderprogramm regional agreement on medical training and clinical research (ALF)
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Karolinska Institutet |
    1000 Förderprogramm regional agreement on medical training and clinical research (ALF)
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer Swedish Foundation for Strategic Research (SSF) |
    1000 Förderprogramm -
    1000 Fördernummer -
  5. 1000 joinedFunding-child
    1000 Förderer Knut and Alice Wallenberg Foundation |
    1000 Förderprogramm -
    1000 Fördernummer -
  6. 1000 joinedFunding-child
    1000 Förderer Carlsberg Foundation |
    1000 Förderprogramm -
    1000 Fördernummer -
  7. 1000 joinedFunding-child
    1000 Förderer Lundbeck Foundation |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6406233.rdf
1000 Erstellt am 2018-01-08T17:54:47.346+0100
1000 Erstellt von 218
1000 beschreibt frl:6406233
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Wed Jun 20 13:05:23 CEST 2018
1000 Objekt bearb. Wed Jun 20 13:05:22 CEST 2018
1000 Vgl. frl:6406233
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406233 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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