WeightNameValue
1000 Titel
  • Application of disease-associated differentially expressed genes – Mining for functional candidate genes for mastitis resistance in cattle
1000 Autor/in
  1. Schwerin, Manfred |
  2. Czernek-Schäfer, Diana |
  3. Kata, Srinivas R. |
  4. Womack, James E. |
  5. Pareek, Ravi |
  6. Pareek, Chandra |
  7. Walawski, Krzysztof |
  8. Brunner, Roland M. |
  9. Goldammer, Tom |
1000 Erscheinungsjahr 2003
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2003-06-15
1000 Erschienen in
1000 Quellenangabe
  • 35(Suppl 1): S19–S34
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3231760/?tool=pmcentrez |
  • http://doi.org/10.1186/1297-9686-35-S1-S19 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • In this study the mRNA differential display method was applied to identify mastitis-associated expressed DNA sequences based on different expression patterns in mammary gland samples of non-infected and infected udder quarters of a cow. In total, 704 different cDNA bands were displayed in both udder samples. Five hundred-and-thirty two bands, (75.6%) were differentially displayed. Ninety prominent cDNA bands were isolated, re-amplified, cloned and sequenced resulting in 87 different sequences. Amongst the 19 expressed sequence tags showing a similarity with previously described genes, the majority of these sequences exhibited homology to protein kinase encoding genes (26.3%), to genes involved in the regulation of gene expression (26.3%), to growth and differentiation factor encoding genes (21.0%) and to immune response or inflammation marker encoding genes (21.0%). These sequences were shown to have mastitis-associated expression in the udder samples of animals with and without clinical mastitis by quantitative RT-PCR. They were mapped physically using a bovine-hamster somatic cell hybrid panel and a 5000 rad bovine whole genome radiation hybrid panel. According to their localization in QTL regions based on an established integrated marker/gene-map and their disease-associated expression, four genes (AHCY, PRKDC, HNRPU, OSTF1) were suggested as potentially involved in mastitis defense.
1000 Sacherschließung
lokal RH mapping
lokal expressed sequence tag
lokal mastitis
lokal cattle
lokal gene expression
1000 Fachgruppe
  1. Agrarwissenschaften |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/U2Nod2VyaW4sIE1hbmZyZWQ=|https://frl.publisso.de/adhoc/creator/Q3plcm5lay1TY2jDpGZlciwgRGlhbmE=|https://frl.publisso.de/adhoc/creator/S2F0YSwgU3Jpbml2YXMgUi4=|https://frl.publisso.de/adhoc/creator/V29tYWNrLCBKYW1lcyBFLg==|https://frl.publisso.de/adhoc/creator/UGFyZWVrLCBSYXZp|https://frl.publisso.de/adhoc/creator/UGFyZWVrLCBDaGFuZHJh|https://frl.publisso.de/adhoc/creator/V2FsYXdza2ksIEtyenlzenRvZg==|https://frl.publisso.de/adhoc/creator/QnJ1bm5lciwgUm9sYW5kIE0u|http://orcid.org/0000-0003-1215-5504
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1000 Erstellt am 2018-01-24T13:36:13.803+0100
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1000 Vgl. frl:6406452
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406452 |
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