WeightNameValue
1000 Titel
  • Hexa-histidin tag position influences disulfide structure but not binding behavior of in vitro folded N-terminal domain of rat corticotropin-releasing factor receptor type 2a
1000 Autor/in
  1. Klose, Jana |
  2. Wendt, Norbert |
  3. Kubald, Sybille |
  4. Krause, Eberhard |
  5. Fechner, Klaus |
  6. Beyermann, Michael |
  7. Bienert, Michael |
  8. Rudolph, Rainer |
  9. Rothemund, Sven |
1000 Erscheinungsjahr 2004
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2004-09
1000 Erschienen in
1000 Quellenangabe
  • 13(9): 2470–2475
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2280012/ |
  • https://doi.org/10.1110/ps.04835904 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The oxidative folding, particularly the arrangement of disulfide bonds of recombinant extracellular N-terminal domains of the corticotropin-releasing factor receptor type 2a bearing five cysteines (C2 to C6), was investigated. Depending on the position of a His-tag, two types of disulfide patterns were found. In the case of an N-terminal His-tag, the disulfide bonds C2–C3 and C4–C6 were found, leaving C5 free, whereas the C-terminal position of the His-tag led to the disulfide pattern C2–C5 and C4–C6, and leaving C3 free. The latter pattern is consistent with the disulfide arrangement of the extracellular N-terminal domain of the corticotropin-releasing factor (CRF) receptor type 1, which has six cysteines (C1 to C6) and in which C1 is paired with C3. However, binding data of the two differently disulfide-bridged domains show no significant differences in binding affinities to selected ligands, indicating the importance of the C-terminal portion of the N-terminal receptor domains, particularly the disulfide bond C4–C6 for ligand binding.
1000 Sacherschließung
lokal protein folding
lokal affinity tag
lokal corticotropin-releasing factor receptor
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/S2xvc2UsIEphbmE=|https://frl.publisso.de/adhoc/creator/V2VuZHQsIE5vcmJlcnQ=|https://frl.publisso.de/adhoc/creator/S3ViYWxkLCBTeWJpbGxl|https://frl.publisso.de/adhoc/creator/S3JhdXNlLCBFYmVyaGFyZA==|https://frl.publisso.de/adhoc/creator/RmVjaG5lciwgS2xhdXM=|https://frl.publisso.de/adhoc/creator/QmV5ZXJtYW5uLCBNaWNoYWVs|https://frl.publisso.de/adhoc/creator/QmllbmVydCwgTWljaGFlbA==|https://frl.publisso.de/adhoc/creator/UnVkb2xwaCwgUmFpbmVy|https://frl.publisso.de/adhoc/creator/Um90aGVtdW5kLCBTdmVu
1000 Objektart article
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1000 @id frl:6406506.rdf
1000 Erstellt am 2018-01-25T19:18:56.420+0100
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1000 Zuletzt bearbeitet Wed Aug 01 11:53:34 CEST 2018
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1000 Vgl. frl:6406506
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406506 |
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