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1000 Titel
  • Facilitating identification of minimal protein binding domains by cross-linking mass spectrometry
1000 Autor/in
  1. Liu, Qingyang |
  2. Remmelzwaal, Sanne |
  3. Heck, Albert J. R. |
  4. Liu, Fan |
  5. Akhmanova, Anna |
1000 Erscheinungsjahr 2017
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-10-18
1000 Erschienen in
1000 Quellenangabe
  • 7:13453
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2017
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1038/s41598-017-13663-y |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647383/ |
1000 Ergänzendes Material
  • https://www.nature.com/articles/s41598-017-13663-y#Sec13 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Characterization of protein interaction domains is crucial for understanding protein functions. Here we combine cross-linking mass spectrometry (XL-MS) with deletion analysis to accurately locate minimal protein interaction domains. As a proof of concept, we investigated in detail the binding interfaces of two protein assemblies: the complex formed by MICAL3, ELKS and Rab8A, which is involved in exocytosis, and the complex of SLAIN2, CLASP2 and ch-TOG, which controls microtubule dynamics. We found that XL-MS provides valuable information to efficiently guide the design of protein fragments that are essential for protein interaction. However, we also observed a number of cross-links between polypeptide regions that were dispensable for complex formation, especially among intrinsically disordered sequences. Collectively, our results indicate that XL-MS, which renders distance restrains of linked residue pairs, accelerates the characterization of protein binding regions in combination with other biochemical approaches.
1000 Sacherschließung
lokal Proteins
lokal Membrane trafficking
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/TGl1LCBRaW5neWFuZyAg|https://frl.publisso.de/adhoc/creator/UmVtbWVsendhYWwsIFNhbm5l|https://frl.publisso.de/adhoc/creator/SGVjaywgQWxiZXJ0IEouIFIu|https://frl.publisso.de/adhoc/creator/TGl1LCBGYW4=|http://orcid.org/0000-0002-9048-8614
1000 Förderer
  1. Netherlands Organization for Scientific Research |
1000 Fördernummer
  1. CW ECHO 711.011.005; 184.032.201
1000 Förderprogramm
  1. Proteins@Work; National Roadmap Large-scale Research Facilities of the Netherlands
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Netherlands Organization for Scientific Research |
    1000 Förderprogramm Proteins@Work; National Roadmap Large-scale Research Facilities of the Netherlands
    1000 Fördernummer CW ECHO 711.011.005; 184.032.201
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6406700.rdf
1000 Erstellt am 2018-02-12T13:55:22.272+0100
1000 Erstellt von 241
1000 beschreibt frl:6406700
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Wed Aug 01 12:30:13 CEST 2018
1000 Objekt bearb. Wed Aug 01 12:30:13 CEST 2018
1000 Vgl. frl:6406700
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406700 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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