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1000 Titel
  • Intersectin associates with synapsin and regulates its nanoscale localization and function
1000 Autor/in
  1. Gerth, Fabian |
  2. Maria Jäpel, Maria |
  3. Pechstein, Arndt |
  4. Kochlamazashvili, Gaga |
  5. Lehmann, Martin |
  6. Puchkov, Dmytro |
  7. Onofri, Franco |
  8. Benfenati, Fabio |
  9. Nikonenko, Alexander G. |
  10. Fredrich, Kristin |
  11. Shupliakov, Oleg |
  12. Freund, Christian |
  13. Haucke, Volker |
  14. Maritzen, Tanja |
1000 Erscheinungsjahr 2017
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-10-23
1000 Erschienen in
1000 Quellenangabe
  • 114(45):12057-12062
1000 FRL-Sammlung
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1073/pnas.1715341114 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5692602/ |
1000 Ergänzendes Material
  • http://www.w3.org/1999/02/www.pnas.org/lookup/suppl/doi:10.1073/pnas.1715341114/-/DCSupplemental |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Neurotransmission is mediated by the exocytic release of neurotransmitters from readily releasable synaptic vesicles (SVs) at the active zone. To sustain neurotransmission during periods of elevated activity, release-ready vesicles need to be replenished from the reserve pool of SVs. The SV-associated synapsins are crucial for maintaining this reserve pool and regulate the mobilization of reserve pool SVs. How replenishment of release-ready SVs from the reserve pool is regulated and which other factors cooperate with synapsins in this process is unknown. Here we identify the endocytic multidomain scaffold protein intersectin as an important regulator of SV replenishment at hippocampal synapses. We found that intersectin directly associates with synapsin I through its Src-homology 3 A domain, and this association is regulated by an intramolecular switch within intersectin 1. Deletion of intersectin 1/2 in mice alters the presynaptic nanoscale distribution of synapsin I and causes defects in sustained neurotransmission due to defective SV replenishment. These phenotypes were rescued by wild-type intersectin 1 but not by a locked mutant of intersectin 1. Our data reveal intersectin as an autoinhibited scaffold that serves as a molecular linker between the synapsin-dependent reserve pool and the presynaptic endocytosis machinery.
1000 Sacherschließung
lokal neurotransmission
lokal NMR spectroscopy
lokal multidomain scaffold
lokal synaptic vesicles
lokal intramolecular regulation
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/R2VydGgsIEZhYmlhbg==|https://frl.publisso.de/adhoc/creator/TWFyaWEgSsOkcGVsLCBNYXJpYQ==|https://frl.publisso.de/adhoc/creator/UGVjaHN0ZWluLCBBcm5kdA==|https://frl.publisso.de/adhoc/creator/S29jaGxhbWF6YXNodmlsaSwgR2FnYQ==|https://frl.publisso.de/adhoc/creator/TGVobWFubiwgTWFydGlu|https://frl.publisso.de/adhoc/creator/UHVjaGtvdiwgRG15dHJv|https://frl.publisso.de/adhoc/creator/T25vZnJpLCBGcmFuY28=|https://frl.publisso.de/adhoc/creator/QmVuZmVuYXRpLCBGYWJpbw==|https://frl.publisso.de/adhoc/creator/Tmlrb25lbmtvLCBBbGV4YW5kZXIgRy4=|https://frl.publisso.de/adhoc/creator/RnJlZHJpY2gsIEtyaXN0aW4=|https://frl.publisso.de/adhoc/creator/U2h1cGxpYWtvdiwgT2xlZw==|https://frl.publisso.de/adhoc/creator/RnJldW5kLCBDaHJpc3RpYW4=|http://orcid.org/0000-0003-3119-6993|http://orcid.org/0000-0003-4993-0340
1000 Label
1000 Förderer
  1. German Research Foundation |
  2. Telethon-Italy |
  3. CARIPLO |
  4. Italian Ministry of Health |
1000 Fördernummer
  1. SFB958/A07; SFB958/A01; NeuroCure Cluster of Excellence Exc-257
  2. GGP13033
  3. 2013-0879
  4. RF 2013
1000 Förderprogramm
  1. -
  2. -
  3. -
  4. -
1000 Dateien
  1. Intersectin associates with synapsin and regulates its nanoscale localization and function
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Research Foundation |
    1000 Förderprogramm -
    1000 Fördernummer SFB958/A07; SFB958/A01; NeuroCure Cluster of Excellence Exc-257
  2. 1000 joinedFunding-child
    1000 Förderer Telethon-Italy |
    1000 Förderprogramm -
    1000 Fördernummer GGP13033
  3. 1000 joinedFunding-child
    1000 Förderer CARIPLO |
    1000 Förderprogramm -
    1000 Fördernummer 2013-0879
  4. 1000 joinedFunding-child
    1000 Förderer Italian Ministry of Health |
    1000 Förderprogramm -
    1000 Fördernummer RF 2013
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6406704.rdf
1000 Erstellt am 2018-02-13T11:17:25.431+0100
1000 Erstellt von 241
1000 beschreibt frl:6406704
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Thu Jan 30 21:16:21 CET 2020
1000 Objekt bearb. Mon May 28 10:20:16 CEST 2018
1000 Vgl. frl:6406704
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406704 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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