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1000 Titel
  • A novel nitroreductase-enhanced MRI contrast agent and its potential application in bacterial imaging
1000 Autor/in
  1. Liu, Yun |
  2. Zhang, Leilei |
  3. Nazare, Marc |
  4. Yao, Qingqiang |
  5. Hu, Hai-Yu |
1000 Erscheinungsjahr 2017
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-12-06
1000 Erschienen in
1000 Quellenangabe
  • In Press
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2017
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1016/j.apsb.2017.11.001 |
1000 Ergänzendes Material
  • https://www.sciencedirect.com/science/article/pii/S2211383517306007#s0115 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Nitroreductases (NTRs) are known to be able to metabolize nitro-substituted compounds in the presence of reduced nicotinamide adenine dinucleotide (NADH) as an electron donor. NTRs are present in a wide range of bacterial genera and, to a lesser extent, in eukaryotes hypoxic tumour cells and tumorous tissues, which makes it an appropriate biomarker for an imaging target to detect the hypoxic status of cancer cells and potential bacterial infections. To evaluate the specific activation level of NTR, great efforts have been devoted to the development of fluorescent probes to detect NTR activities using fluorogenic methods to probe its behaviour in a cellular context; however, NTR-responsive MRI contrast agents are still by far underexplored. In this study, para-nitrobenzyl substituted T1-weighted magnetic resonance imaging (MRI) contrast agent Gd-DOTA-PNB (probe 1) has been designed and explored for the possible detection of NTR. Our experimental results show that probe 1 could serve as an MRI-enhanced contrast agent for monitoring NTR activity. The in vitro response and mechanism of the NTR catalysed reduction of probe 1 have been investigated through LC–MS and MRI. Para-nitrobenzyl substituted probe 1 was catalytically reduced by NTR to the intermediate para-aminobenzyl substituted probe which then underwent a rearrangement elimination reaction to Gd-DOTA, generating the enhanced T1-weighted MR imaging. Further, LC–MS and MRI studies of living Escherichia coli have confirmed the NTR activity detection ability of probe 1 at a cellular level. This method may potentially be used for the diagnosis of bacterial infections.
1000 Sacherschließung
lokal Bacterial infection
lokal Nitroreductase
lokal Smart imaging probes
lokal MRI contrast agent
lokal Bacterial imaging
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/TGl1LCBZdW4=|https://frl.publisso.de/adhoc/creator/WmhhbmcsIExlaWxlaQ==|https://frl.publisso.de/adhoc/creator/TmF6YXJlLCBNYXJj|https://frl.publisso.de/adhoc/creator/WWFvLCBRaW5ncWlhbmc=|https://frl.publisso.de/adhoc/creator/SHUsIEhhaS1ZdQ==
1000 Label
1000 Förderer
  1. Sino-German Center for Research Promotion |
  2. Peking Union Medical College (PUMC) |
  3. Shandong Academiy of Medical Sciences |
1000 Fördernummer
  1. GZ 1271
  2. 3332016056
  3. -
1000 Förderprogramm
  1. -
  2. Youth Fund
  3. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Sino-German Center for Research Promotion |
    1000 Förderprogramm -
    1000 Fördernummer GZ 1271
  2. 1000 joinedFunding-child
    1000 Förderer Peking Union Medical College (PUMC) |
    1000 Förderprogramm Youth Fund
    1000 Fördernummer 3332016056
  3. 1000 joinedFunding-child
    1000 Förderer Shandong Academiy of Medical Sciences |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6406716.rdf
1000 Erstellt am 2018-02-14T11:16:06.357+0100
1000 Erstellt von 241
1000 beschreibt frl:6406716
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet Thu Jan 30 21:17:04 CET 2020
1000 Objekt bearb. Thu May 24 11:15:31 CEST 2018
1000 Vgl. frl:6406716
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406716 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
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