fpls-07-00214.pdf 2,09MB
1000 Titel
  • Reducing Cytoplasmic Polyamine Oxidase Activity in Arabidopsis Increases Salt and Drought Tolerance by Reducing Reactive Oxygen Species Production and Increasing Defense Gene Expression
1000 Autor/in
  1. Sagor, G. H. M. |
  2. Zhang, Siyuan |
  3. Kojima, Seiji |
  4. Simm, Stefan |
  5. Berberich, Thomas |
  6. Kusano, Tomonobu |
1000 Erscheinungsjahr 2016
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-02-29
1000 Erschienen in
1000 Quellenangabe
  • 7:214
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • |
  • |
1000 Ergänzendes Material
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The link between polyamine oxidases (PAOs), which function in polyamine catabolism, and stress responses remains elusive. Here, we address this issue using Arabidopsis pao mutants in which the expression of the five PAO genes is knocked-out or knocked-down. As the five single pao mutants and wild type (WT) showed similar response to salt stress, we tried to generate the mutants that have either the cytoplasmic PAO pathway (pao1 pao5) or the peroxisomal PAO pathway (pao2 pao3 pao4) silenced. However, the latter triple mutant was not obtained. Thus, in this study, we used two double mutants, pao1 pao5 and pao2 pao4. Of interest, pao1 pao5 mutant was NaCl- and drought-tolerant, whereas pao2 pao4 showed similar sensitivity to those stresses as WT. To reveal the underlying mechanism of salt tolerance, further analyses were performed. Na uptake of the mutant (pao1 pao5) decreased to 75% of WT. PAO activity of the mutant was reduced to 62% of WT. The content of reactive oxygen species (ROS) such as hydrogen peroxide, a reaction product of PAO action, and superoxide anion in the mutant became 81 and 72% of the levels in WT upon salt treatment. The mutant contained 2.8-fold higher thermospermine compared to WT. Moreover, the mutant induced the genes of salt overly sensitive-, abscisic acid (ABA)-dependent- and ABA-independent- pathways more strongly than WT upon salt treatment. The results suggest that the Arabidopsis plant silencing cytoplasmic PAOs shows salinity tolerance by reducing ROS production and strongly inducing subsets of stress-responsive genes under stress conditions.
1000 Sacherschließung
lokal drought stress
lokal salinity stress
lokal reactive oxygen species
lokal polyamine oxidase
lokal Arabidopsis thaliana
lokal loss-of-function mutant
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
1000 Label
1000 Förderer
  1. Japan Society for the Promotion of Science (JSPS) |
1000 Fördernummer
  1. 15K14705; 2604081
1000 Förderprogramm
  1. postdoctoral fellowship for foreign researchers
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Japan Society for the Promotion of Science (JSPS) |
    1000 Förderprogramm postdoctoral fellowship for foreign researchers
    1000 Fördernummer 15K14705; 2604081
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6408545.rdf
1000 Erstellt am 2018-06-25T15:32:41.060+0200
1000 Erstellt von 270
1000 beschreibt frl:6408545
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet Tue Jan 05 09:48:17 CET 2021
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1000 Vgl. frl:6408545
1000 Oai Id
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