Download
fphys-09-00172.pdf 1,44MB
WeightNameValue
1000 Titel
  • Agouti Signaling Protein and Its Receptors as Potential Molecular Markers for Intramuscular and Body Fat Deposition in Cattle
1000 Autor/in
  1. Liu, Yinuo |
  2. Albrecht, Elke |
  3. Schering, Lisa |
  4. Kuehn, Christa |
  5. Yang, Runjun |
  6. Zhao, Zhihui |
  7. Maak, Steffen |
1000 Erscheinungsjahr 2018
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2018-03-06
1000 Erschienen in
1000 Quellenangabe
  • 9:172
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2018
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.3389/fphys.2018.00172 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845533/ |
1000 Ergänzendes Material
  • https://www.frontiersin.org/articles/10.3389/fphys.2018.00172/full#h10 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Transcriptome analyses of bovine muscle tissue differing in intramuscular fat (IMF) content identified agouti signaling protein (ASIP) as a promising candidate gene for fat deposition. The protein is secreted from adipocytes and may serve as a signaling molecule in cross-talk between adipocytes and muscle fibers or other cells. Known receptors for ASIP are the melanocortin receptors (e.g., MC4R) and attractin (ATRN). The present study was conducted to determine relationships between the expression of ASIP and its receptors in different bovine tissues with fat deposition. Adipose tissues, liver, and longissimus muscle tissue were collected from 246 F2-generation bulls (Charolais × Holstein cross) and gene expression was measured with RT-qPCR. During analysis of subcutaneous fat (SCF) of all bulls, 17 animals were identified with a transposon-derived transcript (Exon2C) inserted in the ASIP gene and dramatically increased ASIP mRNA levels. Significant correlations between normalized mRNA values of SCF and phenotypic traits related to fat deposition were found in bulls without Exon2C. Three retrospectively assigned groups [Exon2C, n = 17; high carcass fat (HCF), n = 20; low carcass fat (LCF), n = 20] were further analyzed to verify expression differences and elucidate molecular reasons. Expression of ASIP could be detected in isolated muscle fibers and adipocytes of Exon2C bulls in contrast to HCF and LCF bulls, indicating ectopic ASIP expression if the transposon is present. Among adipose tissues, highest ASIP mRNA levels were measured in SCF with significantly higher values in HCF compared to LCF bulls (1.6-fold, P < 0.05). However, the protein abundance was below the detection limit in all bulls. Potential ASIP receptors were detected in most investigated tissues. The expression of MC4R was higher and of ATRN was lower in several tissues of LCF compared to HCF bulls, whereas MC1R was not differentially expressed. Bulls of the Exon2C group had lower ATRN mRNA values than HCF and LCF bulls in perirenal fat (PF), but higher (P < 0.05) values in muscle. Receptors were also expressed in tissues where ASIP mRNA was not detected. Consequently, those tissues could be targets for ASIP if it circulates.
1000 Sacherschließung
lokal attractin
lokal body composition
lokal adipokine
lokal melanocortin receptor
lokal bovine
lokal fat deposition
lokal agouti signaling protein
1000 Fachgruppe
  1. Biologie |
  2. Agrarwissenschaften |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/TGl1LCBZaW51bw==|http://orcid.org/0000-0001-5974-1514|https://frl.publisso.de/adhoc/creator/U2NoZXJpbmcsIExpc2E=|https://frl.publisso.de/adhoc/creator/S3VlaG4sIENocmlzdGE=|https://frl.publisso.de/adhoc/creator/WWFuZywgUnVuanVu|https://frl.publisso.de/adhoc/creator/WmhhbywgWmhpaHVp|http://orcid.org/0000-0003-2681-1496
1000 Label
1000 Förderer
  1. China Scholarship Council (CSC) |
  2. H. Wilhelm Schaumann Foundation |
  3. Leibniz Institute for Farm Animal Biology (FBN) |
1000 Fördernummer
  1. 201406170161
  2. -
  3. -
1000 Förderprogramm
  1. -
  2. -
  3. Open Access Fund
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer China Scholarship Council (CSC) |
    1000 Förderprogramm -
    1000 Fördernummer 201406170161
  2. 1000 joinedFunding-child
    1000 Förderer H. Wilhelm Schaumann Foundation |
    1000 Förderprogramm -
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Leibniz Institute for Farm Animal Biology (FBN) |
    1000 Förderprogramm Open Access Fund
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6410261.rdf
1000 Erstellt am 2018-09-18T10:12:07.481+0200
1000 Erstellt von 122
1000 beschreibt frl:6410261
1000 Bearbeitet von 122
1000 Zuletzt bearbeitet Thu Jan 30 19:14:42 CET 2020
1000 Objekt bearb. Tue Sep 18 10:13:23 CEST 2018
1000 Vgl. frl:6410261
1000 Oai Id
  1. oai:frl.publisso.de:frl:6410261 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

View source