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1000 Titel
  • Fast and reliable dissection of porcine parathyroid glands — A protocol for molecular and histological analyses
1000 Autor/in
  1. Oster, Michael |
  2. Keiler, Jonas |
  3. Schulze, Marko |
  4. Reyer, Henry |
  5. Wree, Andreas |
  6. Wimmers, Klaus |
1000 Erscheinungsjahr 2018
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2018-06-21
1000 Erschienen in
1000 Quellenangabe
  • 219:76-81
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2018
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1016/j.aanat.2018.05.007 |
1000 Ergänzendes Material
  • https://www.sciencedirect.com/science/article/pii/S0940960218300827?via%3Dihub#sec0070 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • As calcium and phosphorus are of vital importance for life, physiological activity of the parathyroid glands (PTGs) is crucial to maintain mineral homeostasis and bone mineralization. However, PTG-specific molecular routes in response to environmental factors and intrinsic hormonal responses are not yet fully understood. Since nutrient requirements, pathophysiology and functional genomics of pigs are similar to those of humans, pigs might be a suitable model to study the holistic gene expression and physiological aspects of the parathyroid gland, which could be used in both animal sciences and biomedical research. However, due to their small size and hidden location, the dissection of the PTGs, particularly in pigs, is difficult. Therefore, a protocol for untrained dissectors has been established that allows a fast and reliable identification of the PTGs in domestic pigs. Based on their localization within the cranial thymus near the carotid bifurcation, sampling was verified by histological staining and mRNA expression pattern. Analyses revealed the prominence of parathyroid hormone (PTH)-producing chief cells. Moreover, the copy numbers of PTH differed substantially between the PTGs and their surrounding thymus tissue, as PTH was expressed virtually exclusively in the PTGs. The developed protocol will substantially facilitate a fast and reliable dissection of porcine PTGs which is essential for studies characterizing the molecular mechanisms of parathyroid glands, e.g. when applying new feeding strategies in pigs.
1000 Sacherschließung
lokal Parathyroid histology
lokal Domestic pig anatomy
lokal Parathyroid hormone (PTH)
lokal Chief cells
lokal Dissection guide
lokal Cervical musculature
lokal Thymus
1000 Fachgruppe
  1. Biologie |
  2. Agrarwissenschaften |
  3. Medizin |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/T3N0ZXIsIE1pY2hhZWw=|https://frl.publisso.de/adhoc/creator/S2VpbGVyLCBKb25hcw==|https://frl.publisso.de/adhoc/creator/U2NodWx6ZSwgTWFya28=|http://orcid.org/0000-0001-6470-0434|https://frl.publisso.de/adhoc/creator/V3JlZSwgQW5kcmVhcw==|http://orcid.org/0000-0002-9523-6790
1000 Förderer
  1. Leibniz ScienceCampus Phosphorus Research Rostock |
  2. European Research Area Network (ERA-NET) |
  3. Sustainable Animal Production (SusAn) |
  4. Leibniz Institute for Farm Animal Biology (FBN) |
  5. Leibniz Association |
1000 Fördernummer
  1. -
  2. -
  3. 2817ERA02D
  4. -
  5. -
1000 Förderprogramm
  1. -
  2. -
  3. PEGaSus project
  4. Open Access Fund
  5. Open Access Fund
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Leibniz ScienceCampus Phosphorus Research Rostock |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer European Research Area Network (ERA-NET) |
    1000 Förderprogramm -
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Sustainable Animal Production (SusAn) |
    1000 Förderprogramm PEGaSus project
    1000 Fördernummer 2817ERA02D
  4. 1000 joinedFunding-child
    1000 Förderer Leibniz Institute for Farm Animal Biology (FBN) |
    1000 Förderprogramm Open Access Fund
    1000 Fördernummer -
  5. 1000 joinedFunding-child
    1000 Förderer Leibniz Association |
    1000 Förderprogramm Open Access Fund
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6410276.rdf
1000 Erstellt am 2018-09-20T08:21:00.172+0200
1000 Erstellt von 25
1000 beschreibt frl:6410276
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet Thu Sep 20 08:24:27 CEST 2018
1000 Objekt bearb. Thu Sep 20 08:22:53 CEST 2018
1000 Vgl. frl:6410276
1000 Oai Id
  1. oai:frl.publisso.de:frl:6410276 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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