WeightNameValue
1000 Titel
  • Proper Application of Antibodies for Immunohistochemical Detection: Antibody Crimes and How to Prevent Them
1000 Autor/in
  1. Ivell, Richard |
  2. Teerds, Katja |
  3. Hoffman, Gloria E. |
1000 Erscheinungsjahr 2014
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2014-03-01
1000 Erschienen in
1000 Quellenangabe
  • 155(3): 676-687
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3929726/ |
  • https://dx.doi.org/10.1210/en.2013-1971 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • For several decades antibodies raised against specific proteins, peptides, or peptide epitopes have proven to be versatile and very powerful tools to demonstrate molecular identity in cells and tissues. New techniques of immunohistochemistry and immunofluorescence have improved both the optical resolution of such protein identification as well as its sensitivity, particularly through the use of amplification methodology. However, this improved sensitivity has also increased the risks of false-positive and false-negative staining and thereby raised the necessity for proper and adequate controls. In this review, the authors draw on many years of experience to illuminate many of the more common errors and problematic issues in immunohistochemistry, and how these may be avoided. A key factor in all of this is that techniques need to be properly documented and especially antibodies and procedures must be adequately described. Antibodies are a valuable and shared resource within the scientific community; it is essential therefore that mistakes involving antibodies and their controls are not perpetuated through inadequate reporting in the literature.
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. http://orcid.org/0000-0001-6513-2109|https://frl.publisso.de/adhoc/creator/VGVlcmRzLCBLYXRqYQ==|https://frl.publisso.de/adhoc/creator/SG9mZm1hbiwgR2xvcmlhIEUu
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft
  2. National Health and Medical Research Council of Australia
  3. Australian Research Council
  4. US National Institutes of Health-National Institute of Neurological Disorders and Stroke
1000 Fördernummer
  1. -
  2. -
  3. -
  4. -
1000 Förderprogramm
  1. -
  2. -
  3. -
  4. -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6410422.rdf
1000 Erstellt am 2018-10-08T13:02:38.406+0200
1000 Erstellt von 122
1000 beschreibt frl:6410422
1000 Bearbeitet von 122
1000 Zuletzt bearbeitet Thu Jan 30 16:54:33 CET 2020
1000 Objekt bearb. Wed Oct 17 09:46:33 CEST 2018
1000 Vgl. frl:6410422
1000 Oai Id
  1. oai:frl.publisso.de:frl:6410422 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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