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WeightNameValue
1000 Titel
  • TRE5-A retrotransposition profiling reveals putative RNA polymerase III transcription complex binding sites on the Dictyostelium extrachromosomal rDNA element
1000 Autor/in
  1. Spaller, Thomas |
  2. Groth, Marco |
  3. Glöckner, Gernot |
  4. Winckler, Thomas |
1000 Erscheinungsjahr 2017
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-04-13
1000 Erschienen in
1000 Quellenangabe
  • 12(4):e0175729
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2017
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1371/journal.pone.0175729 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/28406973/ |
1000 Ergänzendes Material
  • https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0175729#sec017 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The amoeba Dictyostelium discoideum has a haploid genome in which two thirds of the DNA encodes proteins. Consequently, the space available for selfish mobile elements to expand without excess damage to the host genome is limited. The non-long terminal repeat retrotransposon TRE5-A maintains an active population in the D. discoideum genome and apparently adapted to this gene-dense environment by targeting positions ~47 bp upstream of tRNA genes that are devoid of protein-coding regions. Because only ~24% of tRNA genes are associated with a TRE5-A element in the reference genome, we evaluated whether TRE5-A retrotransposition is limited to this subset of tRNA genes. We determined that a tagged TRE5-A element (TRE5-Absr) integrated at 384 of 405 tRNA genes, suggesting that expansion of the current natural TRE5-A population is not limited by the availability of targets. We further observed that TRE5-Absr targets the ribosomal 5S gene on the multicopy extrachromosomal DNA element that carries the ribosomal RNA genes, indicating that TRE5-A integration may extend to the entire RNA polymerase III (Pol III) transcriptome. We determined that both natural TRE5-A and cloned TRE5-Absr retrotranspose to locations on the extrachromosomal rDNA element that contain tRNA gene-typical A/B box promoter motifs without displaying any other tRNA gene context. Based on previous data suggesting that TRE5-A targets tRNA genes by locating Pol III transcription complexes, we propose that A/B box loci reflect Pol III transcription complex assembly sites that possess a function in the biology of the extrachromosomal rDNA element.
1000 Sacherschließung
lokal Polymerase chain reaction
lokal Retrotransposons
lokal DNA transcription
lokal Transfer RNA
lokal Dictyostelium discoideum
lokal Genetic loci
lokal Genomic libraries
lokal Sequence motif analysis
1000 Fachgruppe
  1. Medizin |
  2. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/U3BhbGxlciwgVGhvbWFz|https://orcid.org/0000-0002-9199-8990|https://frl.publisso.de/adhoc/creator/R2zDtmNrbmVyLCBHZXJub3Q=|https://orcid.org/0000-0001-7439-3079
1000 Förderer
  1. German Research Foundation (Deutsche Forschungsgemeinschaft (DFG)) |
1000 Fördernummer
  1. WI 1142/10-1
1000 Förderprogramm
  1. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Research Foundation (Deutsche Forschungsgemeinschaft (DFG)) |
    1000 Förderprogramm -
    1000 Fördernummer WI 1142/10-1
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6413787.rdf
1000 Erstellt am 2019-04-04T10:43:46.767+0200
1000 Erstellt von 285
1000 beschreibt frl:6413787
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet Mon Apr 08 13:50:05 CEST 2019
1000 Objekt bearb. Mon Apr 08 13:49:54 CEST 2019
1000 Vgl. frl:6413787
1000 Oai Id
  1. oai:frl.publisso.de:frl:6413787 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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