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WeightNameValue
1000 Titel
  • Cdc45-induced loading of human RPA onto single-stranded DNA
1000 Autor/in
  1. Szambowska, Anna |
  2. Tessmer, Ingrid |
  3. Prus, Piotr |
  4. Schlott, Bernhard |
  5. Pospiech, Helmut |
  6. Grosse, Frank |
1000 Erscheinungsjahr 2017
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-01-18
1000 Erschienen in
1000 Quellenangabe
  • 45(6):3217-3230
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2017
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1093/nar/gkw1364 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/28100698/ |
1000 Ergänzendes Material
  • https://academic.oup.com/nar/article/45/6/3217/2918644#83689627 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Cell division cycle protein 45 (Cdc45) is an essential component of the eukaryotic replicative DNA helicase. We found that human Cdc45 forms a complex with the single-stranded DNA (ssDNA) binding protein RPA. Moreover, it actively loads RPA onto nascent ssDNA. Pull-down assays and surface plasmon resonance studies revealed that Cdc45-bound RPA complexed with ssDNA in the 8–10 nucleotide binding mode, but dissociated when RPA covered a 30-mer. Real-time analysis of RPA-ssDNA binding demonstrated that Cdc45 catalytically loaded RPA onto ssDNA. This placement reaction required physical contacts of Cdc45 with the RPA70A subdomain. Our results imply that Cdc45 controlled stabilization of the 8-nt RPA binding mode, the subsequent RPA transition into 30-mer mode and facilitated an ordered binding to ssDNA. We propose that a Cdc45-mediated loading guarantees a seamless deposition of RPA on newly emerging ssDNA at the nascent replication fork.
1000 Sacherschließung
lokal repair and replication
lokal genome integrity
1000 Fachgruppe
  1. Medizin |
  2. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/U3phbWJvd3NrYSwgQW5uYQ==|https://frl.publisso.de/adhoc/creator/VGVzc21lciwgSW5ncmlk|https://frl.publisso.de/adhoc/creator/UHJ1cywgUGlvdHI=|https://frl.publisso.de/adhoc/creator/U2NobG90dCwgQmVybmhhcmQ=|https://orcid.org/0000-0002-5255-0747|https://frl.publisso.de/adhoc/creator/R3Jvc3NlLCBGcmFuaw==
1000 Label
1000 Förderer
  1. Federal Government of Germany
  2. State of Thuringia
1000 Fördernummer
  1. -
  2. -
1000 Förderprogramm
  1. Funding for open access charge
  2. Funding for open access charge
1000 Dateien
  1. Cdc45-induced loading of human RPA onto single-stranded DNA
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6413798.rdf
1000 Erstellt am 2019-04-04T13:23:00.336+0200
1000 Erstellt von 285
1000 beschreibt frl:6413798
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet Thu Jan 30 17:21:34 CET 2020
1000 Objekt bearb. Fri Apr 05 13:48:23 CEST 2019
1000 Vgl. frl:6413798
1000 Oai Id
  1. oai:frl.publisso.de:frl:6413798 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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