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1000 Titel
  • Development of Standard Operating Procedure (SOP) of Micro-computed tomography (micro-CT) in Pathology
1000 Autor/in
  1. Teplov, Alexei |
  2. Tabata, Kazuhiro |
  3. Fu, Xiujun |
  4. Uraoka, Naohiro |
  5. Roehrl, Michael H. A. |
  6. Ntiamoah, Peter |
  7. Humm, John L. |
  8. Sirintrapun, Sahussapont J. |
  9. Murray, Melissa P. |
  10. Shia, Jinru |
  11. Travis, William D. |
  12. Brogi, Edi |
  13. Hameed, Meera |
  14. Yagi, Yukako |
1000 Erscheinungsjahr 2019
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2019-07-10
1000 Erschienen in
1000 Quellenangabe
  • 5(1):273
1000 Copyrightjahr
  • 2019
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.17629/www.diagnosticpathology.eu-2019-5:273 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Micro-computed tomography (micro-CT) is an emerging technology in the biomedical field and enables us to analyze 3D structures non-destructively and observe these structures in various directions, thus enabling innovation in this area of pathology. However, application of micro-CT for medicine has just started and optimization per purpose has not yet been done. The purpose of this study is to 1) demonstrate the potential utility of micro-CT in pathology; 2) optimize micro-CT imaging technology and develop a standard operating protocol and; 3) investigate whether micro-CT incurs any radiation damage to pathological tissue samples. The samples of fresh tissue, formalin fixed tissue and formalin fixed paraffin-embedded (FFPE) tissue blocks were scanned using a custom-built Nikon Metrology micro-CT system with a variety of parameters then evaluated with histology correlation in detail. Radiation damage to tissue samples was also evaluated. Through our study, we have established the scanning protocol and workflow for each type of sample. For fresh/fixed tissue, the house made polystyrene foam container was most ideal and the scanning time for fresh tissue was six minutes at as shortest, in which it is possible to detect neoplastic lesions in the tissue. In case of FFPE blocks, 10 - 17 hours scanned images were corresponding with histology specimens in 4-10x under the microscope. Regarding radiation damage to protein expression, no impact was found. Micro-CT imaging of tissue specimens can be of clinical utility in fresh, formalin fixed tissues and FFPE blocks We also found many additional valuable uses of micro-CT images in translational research.
1000 Sacherschließung
lokal tumor boundary analysis
lokal virtual microscopy
lokal 3D-reconstruction
lokal Micro-CT imaging
lokal fixation
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/uri/VGVwbG92LCBBbGV4ZWk=|https://frl.publisso.de/adhoc/uri/VGFiYXRhLCBLYXp1aGlybw==|https://frl.publisso.de/adhoc/uri/RnUsIFhpdWp1bg==|https://frl.publisso.de/adhoc/uri/VXJhb2thLCBOYW9oaXJv|https://frl.publisso.de/adhoc/uri/Um9laHJsLCBNaWNoYWVsIEguIEEu|https://frl.publisso.de/adhoc/uri/TnRpYW1vYWgsIFBldGVy|https://frl.publisso.de/adhoc/uri/SHVtbSwgSm9obiBMLg==|https://frl.publisso.de/adhoc/uri/U2lyaW50cmFwdW4sIFNhaHVzc2Fwb250IEou|https://frl.publisso.de/adhoc/uri/TXVycmF5LCBNZWxpc3NhIFAu|https://frl.publisso.de/adhoc/uri/U2hpYSwgSmlucnU=|https://frl.publisso.de/adhoc/uri/VHJhdmlzLCBXaWxsaWFtIEQu|https://frl.publisso.de/adhoc/uri/QnJvZ2ksIEVkaQ==|https://frl.publisso.de/adhoc/uri/SGFtZWVkLCBNZWVyYQ==|https://frl.publisso.de/adhoc/uri/WWFnaSwgWXVrYWtv
1000 Label
1000 Förderer
  1. NikonMetrology NV |
  2. Warren Alpert Foundation |
  3. Memorial Sloan-Kettering Cancer Center |
1000 Fördernummer
  1. -
  2. -
  3. -
1000 Förderprogramm
  1. -
  2. -
  3. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer NikonMetrology NV |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Warren Alpert Foundation |
    1000 Förderprogramm -
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Memorial Sloan-Kettering Cancer Center |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6415237.rdf
1000 Erstellt am 2019-07-17T12:12:44.279+0200
1000 Erstellt von 218
1000 beschreibt frl:6415237
1000 Bearbeitet von 122
1000 Zuletzt bearbeitet Tue Jul 30 15:01:23 CEST 2019
1000 Objekt bearb. Tue Jul 30 14:49:32 CEST 2019
1000 Vgl. frl:6415237
1000 Oai Id
  1. oai:frl.publisso.de:frl:6415237 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
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