ece3.4960.pdf 884,42KB
1000 Titel
  • Application of a SSR‐GBS marker system on investigation of European Hedgehog species and their hybrid zone dynamics
1000 Autor/in
  1. Curto, Manuel |
  2. Winter, Silvia |
  3. Seiter, Anna |
  4. Schmid, Lukas |
  5. Scheicher, Klaus |
  6. Barthel, Leon M.F. |
  7. Plass, Jürgen |
  8. Meimberg, Harald |
1000 Erscheinungsjahr 2019
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2019-02-14
1000 Erschienen in
1000 Quellenangabe
  • 9(5):2814-2832
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2019
1000 Lizenz
1000 Verlagsversion
  • |
  • |
1000 Ergänzendes Material
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • By applying second‐generation sequencing technologies to microsatellite genotyping, sequence information is produced which can result in high‐resolution population genetics analysis populations and increased replicability between runs and laboratories. In the present study, we establish an approach to study the genetic structure patterns of two European hedgehog species Erinaceaus europaeus and E. roumanicus. These species are usually associated with human settlements and are good models to study anthropogenic impacts on the genetic diversity of wild populations. The short sequence repeats genotyping by sequence (SSR‐GBS) method presented uses amplicon sequences to determine genotypes for which allelic variants can be defined according to both length and single nucleotide polymorphisms (SNPs). To evaluate whether complete sequence information improved genetic structure definition, we compared this information with datasets based solely on length information. We identified a total of 42 markers which were successfully amplified in both species. Overall, genotyping based on complete sequence information resulted in a higher number of alleles, as well as greater genetic diversity and differentiation between species. Additionally, the structure patterns were slightly clearer with a division between both species and some potential hybrids. There was some degree of genetic structure within species, although only in E. roumanicus was this related to geographical distance. The statistically significant results obtained by SSR‐GBS demonstrate that it is superior to electrophoresis‐based methods for SSR genotyping. Moreover, the greater reproducibility and throughput with lower effort which can be obtained with SSR‐GBS and the possibility to include degraded DNA into the analysis, allow for continued relevance of SSR markers during the genomic era.
1000 Sacherschließung
lokal microsatellites
lokal hybridzone
lokal SSR‐GBS
lokal white‐breasted hedgehog
lokal European hedgehog
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
1000 Label
1000 Förderer
  1. Universität für Bodenkultur Wien |
1000 Fördernummer
  1. -
1000 Förderprogramm
  1. “MICSATGEN” project
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Universität für Bodenkultur Wien |
    1000 Förderprogramm “MICSATGEN” project
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6419398.rdf
1000 Erstellt am 2020-03-26T10:42:16.890+0100
1000 Erstellt von 288
1000 beschreibt frl:6419398
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet Mon Mar 30 09:49:11 CEST 2020
1000 Objekt bearb. Mon Mar 30 09:48:46 CEST 2020
1000 Vgl. frl:6419398
1000 Oai Id
  1. |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

View source