Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2

  1. Lau, Yee Ling ORCID logo
  2. Ismail, Ilyiana
  3. Mustapa, Nur Izati
  4. Lai, Meng Yee
  5. Tuan Soh, Tuan Suhaila
  6. Hassan, Afifah
  7. Peariasamy, Kalaiarasu M.
  8. Lee, Yee Leng ORCID logo
  9. Chong, Yoong Min
  10. Sam, I-Ching
  11. Goh, Pik Pin

Download
peerj-9278.pdf 2,35MB
WeightNameValue
1000 Titel
  • Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2
1000 Autor/in
  1. Lau, Yee Ling |
  2. Ismail, Ilyiana |
  3. Mustapa, Nur Izati |
  4. Lai, Meng Yee |
  5. Tuan Soh, Tuan Suhaila |
  6. Hassan, Afifah |
  7. Peariasamy, Kalaiarasu M. |
  8. Lee, Yee Leng |
  9. Chong, Yoong Min |
  10. Sam, I-Ching |
  11. Goh, Pik Pin |
1000 Erscheinungsjahr 2020
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2020-06-03
1000 Erschienen in
1000 Quellenangabe
  • 8:e9278
1000 Copyrightjahr
  • 2020
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.7717/peerj.9278 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275676/ |
1000 Ergänzendes Material
  • https://peerj.com/articles/9278/#supplemental-information |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: Highly sensitive real-time reverse transcription polymerase chain reaction (RT-qPCR) methods have been developed for the detection of SARS-CoV-2. However, they are costly. Loop-mediated isothermal amplification (LAMP) assay has emerged as a novel alternative isothermal amplification method for the detection of nucleic acid. METHODS: A rapid, sensitive and specific real-time reverse transcription LAMP (RT-LAMP) assay was developed for SARS-CoV-2 detection. RESULTS: This assay detected one copy/reaction of SARS-CoV-2 RNA in 30 min. Both the clinical sensitivity and specificity of this assay were 100%. The RT-LAMP showed comparable performance with RT-qPCR. Combining simplicity and cost-effectiveness, this assay is therefore recommended for use in resource resource-limited settings.
1000 Sacherschließung
gnd 1206347392 COVID-19
lokal Rapid detection
lokal Diagnosis
lokal RT-LAMP
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0002-2037-2165|https://frl.publisso.de/adhoc/uri/SXNtYWlsLCBJbHlpYW5h|https://frl.publisso.de/adhoc/uri/TXVzdGFwYSwgTnVyIEl6YXRp|https://frl.publisso.de/adhoc/uri/TGFpLCBNZW5nIFllZQ==|https://frl.publisso.de/adhoc/uri/VHVhbiBTb2gsIFR1YW4gU3VoYWlsYQ==|https://frl.publisso.de/adhoc/uri/SGFzc2FuLCBBZmlmYWg=|https://frl.publisso.de/adhoc/uri/UGVhcmlhc2FteSwgS2FsYWlhcmFzdSBNLg==|https://orcid.org/0000-0001-6944-3836|https://frl.publisso.de/adhoc/uri/Q2hvbmcsIFlvb25nIE1pbg==|https://frl.publisso.de/adhoc/uri/U2FtLCBJLUNoaW5n|https://frl.publisso.de/adhoc/uri/R29oLCBQaWsgUGlu
1000 (Academic) Editor
1000 Label
1000 Förderer
  1. Defense Threat Reduction Agency |
  2. Ministry of Higher Education, Malaysia |
1000 Fördernummer
  1. HDTRA1-17-1-0027
  2. UM.C/625/1/HIR/MOHE/CHAN/14/3
1000 Förderprogramm
  1. Broad Agency Announcement HDTRA1-6
  2. High Impact Research
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Defense Threat Reduction Agency |
    1000 Förderprogramm Broad Agency Announcement HDTRA1-6
    1000 Fördernummer HDTRA1-17-1-0027
  2. 1000 joinedFunding-child
    1000 Förderer Ministry of Higher Education, Malaysia |
    1000 Förderprogramm High Impact Research
    1000 Fördernummer UM.C/625/1/HIR/MOHE/CHAN/14/3
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6421416.rdf
1000 Erstellt am 2020-06-18T15:24:03.900+0200
1000 Erstellt von 122
1000 beschreibt frl:6421416
1000 Bearbeitet von 122
1000 Zuletzt bearbeitet 2020-06-18T15:49:11.252+0200
1000 Objekt bearb. Thu Jun 18 15:48:40 CEST 2020
1000 Vgl. frl:6421416
1000 Oai Id
  1. oai:frl.publisso.de:frl:6421416 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

View source