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1000 Titel
  • Cardiomyocyte Contractility and Autophagy in a Premature Senescence Model of Cardiac Aging
1000 Autor/in
  1. Häseli, Steffen |
  2. Deubel, Stefanie |
  3. Jung, Tobias |
  4. Grune, Tilman |
  5. Ott, Christiane |
1000 Erscheinungsjahr 2020
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2020-04-15
1000 Erschienen in
1000 Quellenangabe
  • 2020:8141307
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2020
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1155/2020/8141307 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180990/ |
1000 Ergänzendes Material
  • https://www.hindawi.com/journals/omcl/2020/8141307/#supplementary-materials |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Globally, cardiovascular diseases are the leading cause of death in the aging population. While the clinical pathology of the aging heart is thoroughly characterized, underlying molecular mechanisms are still insufficiently clarified. The aim of the present study was to establish an in vitro model system of cardiomyocyte premature senescence, culturing heart muscle cells derived from neonatal C57Bl/6J mice for 21 days. Premature senescence of neonatal cardiac myocytes was induced by prolonged culture time in an oxygen-rich postnatal environment. Age-related changes in cellular function were determined by senescence-associated β-galactosidase activity, increasing presence of cell cycle regulators, such as p16, p53, and p21, accumulation of protein aggregates, and restricted proteolysis in terms of decreasing (macro-)autophagy. Furthermore, the culture system was functionally characterized for alterations in cell morphology and contractility. An increase in cellular size associated with induced expression of atrial natriuretic peptides demonstrated a stress-induced hypertrophic phenotype in neonatal cardiomyocytes. Using the recently developed analytical software tool Myocyter, we were able to show a spatiotemporal constraint in spontaneous contraction behavior during cultivation. Within the present study, the 21-day culture of neonatal cardiomyocytes was defined as a functional model system of premature cardiac senescence to study age-related changes in cardiomyocyte contractility and autophagy.
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0002-8933-3236|https://frl.publisso.de/adhoc/uri/RGV1YmVsLCBTdGVmYW5pZQ==|https://orcid.org/0000-0002-9159-8444|https://orcid.org/0000-0003-4775-9973|https://orcid.org/0000-0002-3199-0655
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
  2. Brandenburger Staatsministerium für Wissenschaft, Forschung und Kultur |
  3. Deutsches Zentrum für Herz-Kreislaufforschung |
  4. Bundesministerium für Bildung und Forschung |
  5. German Institute of Human Nutrition Potsdam-Rehbrücke (DIfE) |
1000 Fördernummer
  1. 1240/22-1
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1000 Förderprogramm
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1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer 1240/22-1
  2. 1000 joinedFunding-child
    1000 Förderer Brandenburger Staatsministerium für Wissenschaft, Forschung und Kultur |
    1000 Förderprogramm -
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Deutsches Zentrum für Herz-Kreislaufforschung |
    1000 Förderprogramm -
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer Bundesministerium für Bildung und Forschung |
    1000 Förderprogramm -
    1000 Fördernummer -
  5. 1000 joinedFunding-child
    1000 Förderer German Institute of Human Nutrition Potsdam-Rehbrücke (DIfE) |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6426878.rdf
1000 Erstellt am 2021-04-16T09:54:59.566+0200
1000 Erstellt von 25
1000 beschreibt frl:6426878
1000 Bearbeitet von 16
1000 Zuletzt bearbeitet Fri Apr 16 12:02:25 CEST 2021
1000 Objekt bearb. Fri Apr 16 12:02:24 CEST 2021
1000 Vgl. frl:6426878
1000 Oai Id
  1. oai:frl.publisso.de:frl:6426878 |
1000 Sichtbarkeit Metadaten public
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