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1000 Titel
  • Pattern of circulating SARS‐CoV‐2‐specific antibody‐secreting and memory B‐cell generation in patients with acute COVID‐19
1000 Autor/in
  1. Byazrova, Maria |
  2. Yusubalieva, Gaukhar |
  3. Spiridonova, Anna |
  4. Efimov, Grigory |
  5. Mazurov, Dmitriy |
  6. Baranov, Konstantin |
  7. Baklaushev, Vladimir |
  8. Filatov, Alexander |
1000 Erscheinungsjahr 2021
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2021-01-31
1000 Erschienen in
1000 Quellenangabe
  • 10(2):e1245
1000 Copyrightjahr
  • 2021
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1002/cti2.1245 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848539/ |
1000 Ergänzendes Material
  • https://onlinelibrary.wiley.com/doi/10.1002/cti2.1245#support-information-section |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • OBJECTIVES: To predict the spread of coronavirus disease (COVID‐19), information regarding the immunological memory for disease‐specific antigens is necessary. The possibility of reinfection, as well as the efficacy of vaccines for COVID‐19 that are currently under development, will largely depend on the quality and longevity of immunological memory in patients. To elucidate the process of humoral immunity development, we analysed the generation of plasmablasts and virus receptor‐binding domain (RBD)‐specific memory B (Bmem) cells in patients during the acute phase of COVID‐19. METHODS: The frequencies of RBD‐binding plasmablasts and RBD‐specific antibody‐secreting cells (ASCs) in the peripheral blood samples collected from patients with COVID‐19 were measured using flow cytometry and the ELISpot assay. RESULTS: The acute phase of COVID‐19 was characterised by the transient appearance of total as well as RBD‐binding plasmablasts. ELISpot analysis indicated that most patients exhibited a spontaneous secretion of RBD‐specific ASCs in the circulation with good correlation between the IgG and IgM subsets. IL‐21/CD40L stimulation of purified B cells induced the activation and proliferation of Bmem cells, which led to the generation of plasmablast phenotypic cells as well as RBD‐specific ASCs. No correlation was observed between the frequency of Bmem cell‐derived and spontaneous ASCs, suggesting that the two types of ASCs were weakly associated with each other. CONCLUSION: Our findings reveal that SARS‐CoV‐2‐specific Bmem cells are generated during the acute phase of COVID‐19. These findings can serve as a basis for further studies on the longevity of SARS‐CoV‐2‐specific B‐cell memory.
1000 Sacherschließung
gnd 1206347392 COVID-19
lokal memory B cell
lokal plasmablast
lokal antibody-secreting cell
lokal virus-neutralising antibodies
lokal SARS-CoV-2
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/uri/QnlhenJvdmEsIE1hcmlh|https://frl.publisso.de/adhoc/uri/WXVzdWJhbGlldmEsIEdhdWtoYXI=|https://frl.publisso.de/adhoc/uri/U3Bpcmlkb25vdmEsIEFubmE=|https://frl.publisso.de/adhoc/uri/RWZpbW92LCBHcmlnb3J5|https://frl.publisso.de/adhoc/uri/TWF6dXJvdiwgRG1pdHJpeQ==|https://frl.publisso.de/adhoc/uri/QmFyYW5vdiwgS29uc3RhbnRpbg==|https://frl.publisso.de/adhoc/uri/QmFrbGF1c2hldiwgVmxhZGltaXI=|https://orcid.org/0000-0002-6460-9427
1000 Label
1000 Förderer
  1. Russian Science Foundation |
1000 Fördernummer
  1. 19‐15‐00331
1000 Förderprogramm
  1. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Russian Science Foundation |
    1000 Förderprogramm -
    1000 Fördernummer 19‐15‐00331
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6426986.rdf
1000 Erstellt am 2021-04-21T12:25:34.809+0200
1000 Erstellt von 218
1000 beschreibt frl:6426986
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet Mon May 31 08:57:13 CEST 2021
1000 Objekt bearb. Mon May 31 08:56:47 CEST 2021
1000 Vgl. frl:6426986
1000 Oai Id
  1. oai:frl.publisso.de:frl:6426986 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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